两色金鸡菊黄酮类化学成分研究

Flavonoids isolated from capitula of Coreopsis tinctoria

目的研究两色金鸡菊Coreopsis tinctoria头状花序的水提取物化学成分。方法采用常规硅胶、反相ODS及半制备高效液相色谱等进行分离制备,利用各种谱学技术鉴定化合物结构。结果从两色金鸡菊头状花序的水提物中分离得到13个化合物。依据其波谱数据和理化性质,分别鉴定为8,3′,4′-trihydroxyflavone-7-O-β-D-glucopyranoside(1)、6-hydroxyluteolin-7-O-β-D-glucoside(2)、山柰酚(3)、槲皮素-7-O-β-D-葡萄糖苷(4)、(2S)-3′,4′,5,8-tetrahydroxyflavanone-7-O-β-D-glucoside(5)、(2S)-eriodictyol-5-O-β-D-glucoside(6)、紫铆黄素-7-O-β-D-吡喃葡萄糖苷(7)、plathymenin(8)、(Z)-6-O-β-Dglucopyranosyl-6,3′,4′-trihydroxyaurone(9)、5,6,3′,4′-tetrahydroxyaurone(10)、6,3′,4′-trihydroxyaurone(11)、奥卡宁-5′-O-β-D-葡萄糖苷(12)和4′-O-β-D-glucopyranosyl-3,4,2′,4′,5′-pentahydroxychalcon(13)。结论 13个化合物均为首次从该植物中分离得到。

Objective To study the chemical constituents of the water extract of capitula of Coreopsis tinctoria.Methods The chemical constituents from water extract were isolated and prepared by silica gel column,reversed-phase ODS,and pre-HPLC,and their structures were identified according to the physical and chemical properties and spectral data.Results A total of 13compounds were isolated and identified as 8,3',4'-trihydroxyflavone-7-O-β-D-glucopyranoside (1),6-Hydroxyluteolin-7-O-β-D-glucoside (2), kaempferol (3),quercetin-7-O-β-D-glucopyranoside (4),(2S)-3',4',5,8-tetrahydroxyflavanone-7-O-β-D-glucoside (5),(2S)- eriodictyol-5-O-β-D-glucoside (6),butin-7-O-β-D-glucopyranoside (7),plathymenin (8),(Z)-6-O-β-D-glucopyranosyl-6,3',4'- trihydroxyaurone (9),5,6,3',4'-tetrahydroxyaurone (10),6,3',4'-trihydroxyaurone (11),okanin-5'-O-β-D-glucopyranoside (12),and 4'-O-β-D-glucopyranosyl-3,4,2',4',5'-pentahydroxychalcon (13).Conclusion Thirteen compounds are isolated from C.tinctoria for the first time.