摘要
本研究从云南建水石榴园、蒙自石榴园采集的土样中分离筛选到对石榴枯萎病菌有较强拮抗活性的芽孢杆菌,其中菌株B110对石榴枯萎病菌的室内抑制率为65.88%,研究表明,菌株B110能在石榴根际稳定定殖,具有潜在生防价值。结合菌株B110的培养性状、菌落形态观察、生理生化特性分析(Bolog系统)及gyrB基因序列分析,将菌株B110初步鉴定为解淀粉芽孢杆菌Baci-llus amylolique faciens。
In this study,the bacillus with strong antagonism activity to pomegranate wilt pathogen was isolated from the soil samples collected from pomegranate orchards of Jianshui and Mengzi county in Yunnan Province.The indoor inhibition efficiency of bacillus strain B110 to pomegranate wilt pathogen was 65.88%.This bacillus strain B110 can colonize in the rhizosphere soil of pomegranate.It was identified as Bacillus amyloliquefaciens,according to the characters of cultural colonies,morphological observation,biochemical and physiological identification（Biolog carbon source utilization analysis）and gyrBgene sequence analysis.
作者
周银丽
郭建伟
杨伟
胡先奇
ZHOU Yinli;GUO Jianwei;YANG Wei;HU Xianqi(Key Laboratory of Higher Quality and Efficient Cultivation and Safety Control of Crops for,Yunnan Provnce,Honghe University,Mengzi,Yunnan,661100;Commercial College of Honghe University,Mengzi,Yunnan,661100;Key Laboraiory of Agri-biodiversity and Pest Management of Education Ministry of China,Yunnan"Agricultural University,Kunming,650201)
出处
《中国南方果树》
北大核心
2018年第6期1-6,共6页
South China Fruits
基金
云南省应用基础研究项目(2013FZ125)
红河学院科研基金博士专项项目(XJ17B10)
红河学院中青年学术骨干人才计划项目(2015GG0206)
红河学院硕士点植物保护一级学科建设项目资助
关键词
石榴枯萎病菌
生物防治
解淀粉芽孢杆菌
pomegranate wilt pathogen
biological control
Bacillus amyloliquefaciens
