铝染毒对大鼠血清腺苷三磷酸酶活性的影响

Effect of aluminum exposure on activity of serum adenosine triphosphatase in rats

[目的]研究铝染毒对大鼠糖代谢相关指标及腺苷三磷酸酶活性的影响,探讨铝影响机体糖代谢的可能机制。[方法]60只SPF级Wistar雄性大鼠用随机数字表法按体重随机分成4组,即空白对照组和低、中、高剂量染毒组,每组15只。染毒组每组分别给予AlCl3溶液以2、4、8 mg/(kg·d)AlCl3溶液进行腹腔注射,对照组给予腹腔注射等体积生理盐水(2 mL/只)。每天注射1次,全程共30 d。于第10、20、30天每组取5只大鼠检测空腹血糖(FBG)、血清胰岛素(FINS)水平,分光光度法检测血清Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性。[结果]随着时间延长,铝染毒大鼠食欲减退,进食量减少,精神萎靡,体重增长缓慢。FBG在各时点的中剂量组、高剂量组均高于对照组(P<0.05)。FINS于第10、20天的高剂量组高于对照组,在第30天反而低于对照组(P<0.05)。第10、20天,中、高剂量组胰岛素抵抗指数均高于对照组(P<0.05);第30天,低剂量组、中剂量组明显高于对照组(P<0.05)。第20和30天,中、高剂量组胰岛β细胞功能指数明显低于对照组(P<0.05)。Na+-K+-ATPase在各时点的中、高剂量组均低于对照组(P<0.05),Ca2+-Mg2+-ATPase在各时点的高剂量组均低于对照组(P<0.05)。[结论]铝染毒可能通过降低腺苷三磷酸酶活性,减弱机体血糖调节能力。

[Objective ]To test the effects of aluminum exposure on glycometabolism-related indicators and adenosine triphosphatase (ATPase)activity in rats,and explore the possible mechanisms of aluminum exposure on glucose metabolism. [Methods ]A total of 60 SPF Wistar male rats were randomly divided into four groups:one control group and three aluminum exposure groups (low dose,medium dose,and high dose),with 15 rats in each group.The aluminum exposure groups were intraperitoneally injected with 2,4,and 8mg/(kg·d)AlCl3solution,respectively;the control group received intraperitoneal normal saline injection (2mL per rat).The 30-day treatment protocol included intraperitoneal injection once a day.On day 10,20,and 30, five rats in each group were taken to test for levels of fasting blood glucose (FBG)and serum insulin (FINS),and activities of serum Na^+-K^+-ATPase and Ca^2+-Mg^2+-ATPase were detected by spectrophotometry. [Results ]With time prolonged,the rats with aluminum exposure suffered from loss of appetite,reduced food intake,low spirits, and slow weight gain.Compared with the control group,the FBG levels were higher in the middle dose group and the high dose group at each selected time point (P<0.05);The FINS levels in the high dose group were higher than those in the control group on day 10 and 20,but lower on day 30(P<0.05);The HOMA-IR levels in the middle dose group and the high dose group were higher than those in the control group on day 10and 20(P<0.05),and the levels in the low dose group and the middle dose group were higher than those in the control group on day 30(P<0.05).The HOMA-βlevels in the middle dose group and the high dose group were lower than those in the control group on day 20 and 30(P<0.05).The activities of Na^+-K^+-ATPase in the middle dose group and the high dose group were lower than those in the control group at each selected time point (P <0.05).The activity of Ca^2+-Mg^2+-ATPase in the high dose group was lower than that in the control group at each selected time point (P <0.05). [Conclusion ]Aluminum exposure may reduce the body's ability to regulate blood glucose by lowering the activity of ATPase.