氧化锆表面紫外线处理对成骨细胞生物学行为的影响

The influence of ultraviolet treated zirconia with different roughness on the function of osteoblasts

目的:探讨不同粗糙度氧化锆表面经紫外线处理,对小鼠胚胎成骨细胞前体细胞(MC3T3-E1)生物学行为的影响。方法:将氧化锆试件先行抛光和喷砂,处理组用15 W[波长(250±20)nm]灭菌灯对试件照射48 h,分为光滑对照组(S组)、光滑处理组(S-UV组)、粗糙对照组(R组)和粗糙处理组(R-UV组)4组(n=3),将MC3T3-E1细胞分别培养于试件表面。MTT法检测接种3、5 d细胞增殖率,3、7 d时检测细胞分泌碱性磷酸酶(ALP)的活性;茜素红染色培养21 d细胞,检测钙结节形成情况;RT-PCR法检测培养7、14 d骨相关蛋白骨钙素(OCN)、护骨素(OPG)mRNA的表达。结果:紫外线处理组比对照组更利于MC3T3-E1细胞的增殖与矿化,R-UV组与其他组差异明显(P<0.05),7 d组的ALP活性高于3 d组(P<0.05),7 d时R-UV组ALP活性均高于S组和S-UV组(P<0.05),粗糙组间无明显差异。RT-PCR显示,R-UV组OCN及OPG mRNA的表达明显上调(P<0.05)。结论:氧化锆表面经紫外线处理后可促进成骨细胞在其表面增殖、矿化,上调OCN、OPG mRNA的表达。

Objective:To study the effects of UV treatment of different zirconia surfaces on the function of MC3T3-E1osteoblasts. Methods:Disks of zirconia-based materials with smooth(S)and rough(R)surfaces were treated and with and without UV light(15 W)for48h,respectively.MC3T3-E1cells were cultured on zirconia disks and divided into4groups:smooth group(S),UV treated smooth group(S-UV),rough group(R)and UV treated rough group(R-UV).Cell proliferation was assessed by MTT3and5days after culture.Alkaline phosphatase(ALP)activity was determined3and7days after culture.Cell mineralization was observed by Alizarin red dye staining21days after culture.The mRNA expression of OCN and OPG were examined by RT-PCR7and14days after culture.Results:Proliferation and mineralization of MC3T3-E1cells in R-UV group were more than in other groups(P<0.05).R groups showed higher ALP activity than S groups at day7.R-UV treatment increased the mRNA expressions of OPG and OCN in a time-dependent manner(P<0.05).Conclusion:Ultraviolet light-treated zirconia surface may promote the proliferation,mineralization and the expression of OCN and OPG of MC3T3-E1cells.