敲除小鼠生殖细胞Wdr1基因对卵巢功能的影响

Effect of germ cell Wdr1 deletion on the ovarian function of mice

目的探讨敲除小鼠Wdr1基因对其卵巢功能的影响。方法用条件性基因敲除小鼠Wdr1^fl/fl和生殖细胞特异性启动子驱动的Cre重组酶转基因小鼠构建卵母细胞特异性基因敲除小鼠模型。取出生14天、28天和4个月3个时间点的Wdr1 fl/-;Ddx4-Cre小鼠与对照鼠(每个时间点每种1只),处死。取卵巢,拍照、石蜡连续切片及苏木精-伊红染色(Hematoxylin-Eosin staining,HE)处理,观察卵巢体积变化及各级卵泡的数量。结果14天Wdr1^fl/-;Ddx4-Cre小鼠卵巢体积比对照小鼠略小;HE切片镜下可见原始卵泡、初级卵泡、次级卵泡等,较对照鼠略少。28天及4个月Wdr1^fl/-;Ddx4-Cre小鼠卵巢体积明显小于对照小鼠;HE切片镜下可见各级卵泡明显少于对照小鼠。结论敲除小鼠生殖细胞Wdr1基因对其早期卵巢功能略有影响,随着时间的推移,可导致卵巢早衰。

Objective To study the effect of Wdr1 deletion in germ cells on ovarian function of mice. Methods Oocyte-specific gene knockout mouse model was constructed by crossing Wdr 1 fl/fl female mice with Cre recombinase transgenic male mice which was driven by a germ cell-specific promoter.Wdr1 fl/-;Ddx4-Cre mice and control mice were sacrificed at 14 days,28 days and 4 months after birth,whose ovaries were subjected to photography,paraffin sectioning and Hematoxylin-Eosin (HE)staining.The ovarian volume and follicular numbers were recorded at various time points.Results The ovarian volume of Wdr1 fl/-;Ddx4-Cre mice was slightly lower than that of the controls at 14 days. HE staining showed that primordial follicles,primary follicles and secondary follicles were slightly reduced compared with the control mice at 14 days.The ovarian volume of Wdr1 fl/-;Ddx4-Cre mice was significantly lower than that of the control mice at 28 days and 4 months.HE staining showed that all developmental follicles were significantly reduced compared with the control mice.Conclusion Wdr1 gene deletion in germ cells can influence early ovarian function of mice and lead to premature ovarian failure.