IL-17A基因rs3819024和rs8193037单核苷酸多态性与缺血性脑卒中的相关性

Association of single nucleotide polymorphisms of rs3819024and rs8193037loci of IL-17A gene with the risk of ischemic stroke

目的探讨白细胞介素17A(IL-17A)基因启动子区rs3819024和rs8193037位点单核苷酸多态性与缺血性脑卒中发病的相关性。方法用SNaPshot技术和DNA测序法对广西地区392例缺血性脑卒中患者和443名正常对照rs3819024和rs8193037位点进行基因分型,比较两组人群间两位点的遗传差异。结果rs3819024位点基因型、显性模型、隐性模型以及等位基因在两组间的差异均无统计学意义,P值分别为0.150、0.227、0.125、0.594和0.202,OR(95%CI)分别为1.27(0.92~1.74)、1.28(0.86~1.91)、1.27(0.94~1.72)、1.10(0.78~1.54)和1.13(0.94~1.38)。rs8193037位点基因型、显性模型、隐性模型以及等位基因在两组间的差异亦均无统计学意义,P值分别为0.722、0.352、0.863、0.345和0.969,OR(95%CI)值分别为0.94(0.65~1.35)、2.25(0.41~12.35)、0.97(0.68~1.38)、2.27(0.41~12.48)和1.01(0.72~1.40)。结论 IL-17A基因rs3819024和rs8193037位点单核苷酸多态性可能与广西人群缺血性脑卒的遗传易感性无关。

Objective To assess the association of polymorphisms of rs3819024 and rs8193037 loci in the promoter region of IL-17A gene with the risk of ischemic stroke (IS)among ethnic Han Chinese from Guangxi.Methods The polymorphisms of rs3819024and rs8193037loci were detected by a SNaPshot assay and DNA sequencing among 392IS patients and 443healthy controls with matched age and gender.Results The genotypes,dominant model,recessive model,and alleles of rs3819024polymorphisms showed no significant difference between the two groups,with the P values calculated as 0.150,0.227,0.125,0.594 and 0.202,respectively,and OR (95% CI)as 1.27(0.92-1.74),1.28(0.86-1.91),1.27(0.94-1.72), 1.10(0.78-1.54),and 1.13(0.94-1.38),respectively.The genotypes,dominant model,recessive model, and alleles of rs8193037polymorphisms also showed no significant difference between the two groups,with the P values calculated as 0.722,0.352,0.863,0.345 and 0.969,respectively,and OR (95% CI)as 0.94 (0.65-1.35),2.25(0.41-12.35),0.97(0.68-1.38),2.27(0.41-12.48),and 1.01(0.72-1.40), respectively.Conclusion Polymorphisms of the rs3819024 and rs8193037 loci of the IL-17A gene are not associated with the susceptibility to IS among ethnic Han Chinese from Guangxi.