3M Petrifilm^(TM)快速测试片与国标法检测饮料中霉菌和酵母的比较

Comparison of 3M Petrifilm^(TM) Rapid Test Strips and the National Standard Method for Analyzing the Mold and Yeast Species in Beverages

本研究使用3M Petrifilm^(TM)快速霉菌酵母测试片以及国标法同时对某功能维生素饮料样品进行霉菌计数和酵母计数检测,比较两者结果是否具有显著性差异。试验筛选采用白色念珠菌(Candida albicans)、拟热带假丝酵母(Candida tropicalis)、汉逊德巴利酵母(Debaryomyces hansenii)、绿木霉(Trichoderma virens)、炭黑曲霉(Aspergillus carbonarius)5株标准菌株进行试验,分别进行了菌株的预选试验、快速测试片检测的重复性试验和特异性试验,以及快速测试片法和国标法的比较试验。研究结果显示,菌株预选试验所筛选的菌株均能在测试样品中生长良好;重复性试验结果表明第二次结果均在第一次结果可接受范围内;特异性试验中表明快速测试片特异性良好;对比试验中使用两种方法对样品进行霉菌和酵母计数检测,结果使用F检验、t检验进行统计分析,结果表明3M Petrifilm^(TM)测试片法与国标法两种方法总体均值无显著差异。

In this study,3M Petrifilm^TM Rapid Test Strips for yeasts and molds and the national standard method were used in parallel for mold count and yeast count tests for a functional vitamin beverage,to determine whether or not the analysis results obtained by the two methods were significantly different.Five standard strains (Candida albicans,Candida tropicalis,Debaryomyces hansenii,Trichoderma virens and Aspergillus carbonarius)were used for screening.Strain preselection test,repeatability test and specificity test of the rapid test strips,and comparative tests between the rapid test strip method and the national standard method,were performed.The obtained results showed that the strains selected through the preselection test could all grow well in the test samples.Meanwhile,the repeatability test revealed that the results of the second test were within the acceptable range of those of the first test;the specificity test revealed that the rapid test strips method had high specificity.The results of the mold and yeast counts determined by the two comparative methods were statistically analyzed by the F test and t test,and it was found that there was no significant difference between the results obtained by the 3M PetrifilmTM rapid test strip method and the national standard method.