美洲大蠊提取物Ento-B对2,4-二硝基氯苯联合醋酸诱导大鼠溃疡性结肠炎的作用及机制初探

Study on the effect of the treatment of Periplaneta americana extract Ento-B by 2,4-dinitrochlorobenzene combined with acetic acid induced ulcerative colitis in rats

目的研究美洲大蠊提取物Ento-B对2,4-二硝基氯苯联合醋酸诱导的大鼠慢性溃疡性结肠炎的治疗作用,并探讨其初步作用机制。方法采用2,4-二硝基氯苯联合醋酸诱导大鼠溃疡性结肠炎模型,灌肠给予美沙拉嗪(Mesalazine)(400mg·kg-1)和Ento-B(200mg·kg-1、100mg·kg-1、50mg·kg-1)药物治疗,通过测定大鼠疾病活动指数(DAI)、结肠黏膜损伤指数(CMDI)、病理组织学评分(HS),检测血清IL-4、IL-10、TNF-α、MDA、SOD、NOS的表达水平来评价Ento-B对UC大鼠的作用。结果与模型对照组比较,Ento-B各剂量组均能降低UC大鼠的CMDI(P <0. 05)、HS(P <0. 05或P <0. 01)的评分,显著升高UC大鼠血清IL-4、IL-10、SOD的表达(P <0. 01),降低TNF-α、MDA、NOS的含量(P <0. 05或P<0. 01),明显改善UC大鼠结肠的病变程度。结论 Ento-B对DNCB联合醋酸诱导的大鼠溃疡性结肠炎有治疗作用,机理可能与提高UC大鼠IL-4、IL-10、SOD和降低TNF-α、MDA、NOS的表达水平有关。

Objective To study the Periplaneta americana extract Ento -B on the treatment of chronic ulcerative colitis induced by 2,4-dinitrochlorobenzene and acetic acid in rats,and to explore its primary mechanism of action.Methods Using 2,4-dinitrochlorobenzene combined with acetic acid to induce chronic ulcerative colitis (chronic UC)in rats.Enema therapy to give the mesalazine(400mg·kg^-1)and Ento -B(200mg·kg^-1,100mg·kg^-1,50mg·kg^-1),and evaluate the effect according to the disease activity index (DAI)score,colon mucosal injury index (CMDI)score,histopathological score (HS),and the serum levels of IL-4,IL-10,TNF-α,MDA,SOD and NOS.Results Compared with the model control group,all doses of Ento-B could reduce the score of CMDI(P <0.05),HS(P <0.05or P <0.01),significantly increased the expression of IL-4,IL-10,SOD(P <0.01)and decreased the levels of TNF-α,MDA,NOS in serum of UC rats,significantly improve the degree of colon lesions in UC rats.Conclusion Ento -B may play an important role in the treatment of ulcerative colitis induced by UC rats.The mechanism may be related to the increased expression of IL-4,IL-10,SOD and reduced expression of TNF -α, MDA,NOS.