闪式提取猴头菌-地龙生物转化物中抗凝血活性成分的工艺研究

Research on the Process of Homogenate Extraction Anticoagulant Components from Hericium Erinaceus-Pheretima Biotransformation

目的优选闪式提取猴头菌-地龙生物转化物(Hericium erinaceus-Pheretima biotransformation,HD)中抗凝血活性成分工艺参数。方法以活化部分凝血酶时间为评价指标,以水作提取溶剂,提取2次,通过响应面设计试验法,对闪式提取HD中抗凝血活性成分的主要工艺参数进行优化。结果闪式提取HD中抗凝血活性成分的最优工艺条件为:提取2次,第1次提取加水12倍,5 500 r·min^(-1)提取7 min;第2次提取加水10倍,5 500 r·min^(-1)提取7 min。在此工艺条件下所制备提取液的活化部分凝血酶时间均值最高,为(53.42±3.02)s。结论通过优化与验证,首次创立了闪式提取HD中抗凝血活性成分的工艺路线。该工艺提取速度快,提取溶剂无毒,提取设备操作简单、所占空间小,节约资源。

OBJECTIVE To optimize process parameters of homogenate extraction anticoagulant components from Hericium erinaceus-Pheretima biotransformation(HD). METHODS The activated partial thromboplastin time(APTT) was used as the assessment index. Water was selected as extracting solvent, extracted 2 times. These main factors in the process of homogenate extraction anticoagulant components from HD which had effect on the APTT were optimized through response surface methodology. RESULTS The optimum process parameters of homogenate extraction anticoagulant components from HD were as follows: extracted 2 times, the additive quantity of water was 12 times at the first times, the extracting time was 7 min, the revolution was 5 500 r·min-1, the additive quantity of water was 10 times at the second times, the extracting time was 7 min, the revolution was 5 500 r·min-1. Under these conditions, the average value of APTT was the maximum, came up to(53.42±3.02)s. CONCLUSION A new process route of homogenate extraction anticoagulant components from HD has been established by optimization and verification experiment at the first time. The process is faster, healthier, simpler, and the equipment is smaller area occupied and save resources.