超声波刺激粗糙脉胞菌发酵产番茄红素的工艺研究

Study on the technique of ultrasonic stimulation to produce lycopene by Neurospora crassa

超声波对粗糙脉胞菌(Neurospora crassa,N.crassa)发酵产番茄红素的影响和工艺条件进行了研究与优化。在单因素实验的基础上,选取超声起始时间、超声的次数和每次超声时间为自变量,以番茄红素的产量为响应值,采用Box-Behnken试验设计及响应面分析法,对该工艺进行了优化。结果表明:在30℃、110r/min震荡培养至19h开始进行超声(200W、40kHz)处理,每次超声处理6.3min,每隔24h处理一次共处理3次,番茄红素的产量为(29.5062±0.2093)mg/L。

In this paper,the effect of ultrasound on lycopene production by Neurospora crassa was studied and the stimulation conditions were optimized.The single factor experiment combined with Box-Behnken design was used to optimize the ultrasonic stimulation conditions,with the yield of lycopene as evaluation index and the ultrasonic initiation time,ultrasonic treatment frequency and ultrasonic time per treatment as independent variables.The research results showed that the optimum stimulation conditions were.temperature 30℃,shaking speed 100r/min,ultrasonic power 200W,ultrasonic frequency 40kHz,ultrasonic initiation time 19h,ultrasonic treatment once every 24hours and 3times in total,and ultrasonic time 6.3min per treatment .Under the conditions,the yield of lycopene was (29.5062±0.2093)mg/L.