菜用大豆蔗糖转运蛋白基因家族的克隆及表达分析

Gene Clone and Expression Analysis of Sucrose Transporter Gene Family from Vegetable Soybean

蔗糖是高等植物体内碳水化合物长距离运输的主要甚至唯一的形式,蔗糖转运蛋白在蔗糖转运过程中起着极为重要的作用。该研究从菜用大豆闽豆6号中克隆到9个蔗糖转运蛋白基因,分别命名为Gm SUT-1~GmSUT-9,并对其进行生物信息学、系统发育和组织表达分析。结果表明,GmSUTs属于MFS超家族,根据SUT系统进化分析法将GmSUTs划分为SUT1、SUT2和SUT4三个亚族,其中GmSUT-1、GmSUT-2、GmSUT-4、GmSUT-5、GmSUT-6、GmSUT-7和GmSUT-8属于SUT1亚族, GmSUT-9属于SUT2亚族, GmSUT-3属于SUT4亚族。SUTs各亚族成员氨基酸序列的差异性可能导致它们在蔗糖的跨膜转运中扮演不同的角色,其表达模式和生理功能也不同。大豆SUT1亚族中7个基因总的表达趋势相似,花中表达量相对来说最高,而籽粒、叶片、茎和根等组织中表达量则相对较低; SUT2亚族中的GmSUT-9和SUT4亚族中的GmSUT-3在不同组织中的表达量相对来说都较低。该研究初步阐明了菜用大豆蔗糖转运蛋白家族基因的结构及组织表达特点,为进一步开展蔗糖转运蛋白基因调控菜用大豆籽粒发育中蔗糖的积累和分配的研究奠定了基础。

Sucrose is the main or even the only form of long distance transport of carbohydrates in higher plants, and sucrose transporter plays a very important role in the process of sucrose transport. In this study, 9 sucrose transporter genes were cloned from vegetable soybean Mindou 6, named GmSUT-1-GmSUT-9, and their bioinformatics, phylogeny and tissue expression were analyzed. The results showed that GmSUTs belonged to MFS superfamily. According to the SUT phylogenetic analysis, GmSUTs was divided into three subgroups of SUT1, SUT2 and SUT4, of which GmSUT-1, GmSUT-2, GmSUT-4, GmSUT-5, GmSUT-6, GmSUT-7 and GmSUT-8 belonged to subgroup SUT1, GmSUT-9 belonged to subgroup SUT2 and GmSUT-3 belonged to subgroup SUT4. The differences in amino acid sequences of members of SUTs subgroups might lead to different roles in transmembrane transport of sucrose, and their expression patterns and physiological functions were also different. The total expression of 7 genes in the SUT1 subfamily of soybean is similar, the highest expression is in the flower, while the expression of the seeds, leaves, stems and roots is relatively low, and the expression of GmSUT-3 in SUT4 subgroup and Gm SUT-9 in SUT2 subgroup was relatively low. This study preliminarily clarified the structure and tissue expression characteristics of the sugar transporter family gene in vegetable soybean, which laid the foundation for the further development of sucrose transporter gene regulation in the accumulation and distribution of sucrose in the development of vegetable soybean seeds.