抗氧化剂在清除活性氧及提高农杆菌介导转化效率中的应用

The Application of Antioxidant on Removing Reactive Oxygen Species and Enhancing Agrobacterium tumefaciens Mediated Transformation Efficiency

组织细胞褐变坏死、芽逃离和转化顽拗是影响农杆菌介导转化效率的三大因素,研究表明,这些因素均与活性氧(reactive oxygen species, ROS)产生有关,过量ROS会影响植物细胞的全能性。添加抗氧化剂不仅可以减少农杆菌介导转化过程中外植体褐变坏死,而且可以增强农杆菌活力,促进外植体生长,增强农杆菌介导转化效率。该文综述了农杆菌介导转化过程中活性氧的产生及其对转化效率的影响,尤其是新型抗氧化剂硫辛酸在提高农杆菌介导转化效率中的作用。

The browning and necrosis of plant cell and tissues,shoot escape and recalcitrant are three major factors to influence transformation efficiency in the Agrobacterium-mediated transformation process in many plant species.Recent studies had shown that these phenomena were closely related to reactive oxygen species (ROS).Ex- cessive ROS could affect the plant cell totipotency.Addition antioxidants in the tissue culture media could reduce the explant tissue and cells necrosis during Agrobacterium-mediated transformation process,improve the Agrobacterium vitality and promote the explant tissue and cells regeneration.This paper reviewed the ROS production during Agro- bacterium-mediated transformation and its effect on the transformation efficiency,especially the role of new type of antioxidant-lipoic acid to enhance transformation efficiency during Agrobacterium-mediated transformation.