CHO细胞重组抗体表达载体的构建策略及进展

The Construction Strategy and Progress of the Recombinant Antibody Expression Vector in CHO Cells

近年来,重组蛋白被用来治疗多种不同的疾病,其中重组单克隆抗体是增长最快速的一类生物治疗性重组蛋白。中国仓鼠卵巢(Chinese hamster ovary, CHO)细胞是目前最常用的重组抗体生产的宿主细胞。表达载体是重组抗体表达的重要部分,直接影响抗体表达的水平和质量。目前, CHO细胞重组抗体表达载体的构建策略主要有单顺反子表达载体、多启动子表达载体、IRES连接载体、Furin-2A连接载体、抗体融合蛋白等。其中, Furin-2A连接载体由于具有多种优点而逐渐成为表达重组抗体的一种重要策略,尤其是其具有较高的自剪切效率、连接的两个基因表达较为平衡、基因序列短小等优点。该文概括了目前构建CHO细胞重组抗体表达载体的策略、优缺点及其进展。

In recent years, recombinant proteins are used in the treatment of different diseases. Among of them, monoclonal antibodies (mAbs) are currently the fastest growing class of biotherapeutic recombinant proteins. Chinese hamster ovary (CHO) cell is the most commonly host cells for the production of recombinant antibodies. Expression vector is an important part of recombinant antibody expression, which influences the expression level and quality of recombinant antibody. At present, the construction strategies of recombinant monoclonal antibodies expression vectors are mainly multiple-promoter expression vector, IRES-mediated vector, Furin-2A-mediated vector and antibody fusion protein in CHO cells. Among of them, Furin-2A-mediated vector present an effective approach to construct the recombinant protein expression vector with a variety of advantages, especially of its high “self- cleavage” efficiency, good balance of two genes expression and its short gene sequences. In this study, the strategies of constructing recombinant monoclonal antibodies expression vector in CHO cells and its potential advantages, disadvantages and progress are reviewed.