基于多种分析模式构建壮药滇桂艾纳香HPLC指纹图谱

HPLC Fingerprint of Blumea riparia Based on Diversified Analytical Models

目的:建立滇桂艾纳香药材HPLC指纹图谱分析方法。方法:采用HPLC法,色谱柱为Inertsil ODS-3柱(250 mm×4.6 mm,5μm);流动相为甲醇-0.2%磷酸溶液,梯度洗脱;流速1.0 m L/min;柱温25℃;检测波长280nm。建立滇桂艾纳香指纹图谱,并对其进行相似度评价、聚类分析和主成分分析。结果:建立了10批滇桂艾纳香药材的指纹图谱共有模式,标定了15个共有峰,指认了原儿茶酸、绿原酸、咖啡酸和芦丁4个指标性成分。10批药材相似度均>0.9;聚类分析结果将药材聚为3类,主成分分析筛选出影响药材质量的5个主要组分。结论:所建立的指纹图谱及其分析模式稳定可靠,可为滇桂艾纳香的质量控制提供参考。

Objective:To establish the HPLC fingerprint of Blumea riparia.Methods:The HPLC analysis was carried out by Inertsil ODS-3 column(250 mm×4.6 mm,5μm)with mobile phase of methanol-0.2% phosphoric acid in gradient elution,the flow rate was 1.0 mL/min,the column temperature was 25℃ ,and the detection wavelength was 280 nm.The HPLC fingerprint of Blumea riparia was set up.The quality of ten batches samples was evaluated by similarity analysis,cluster analysis and principal component analysis.Results :The HPLC fingerprint of Blumea riparia was established,15 common peaks were calibrated,four of them were identified as protocatechuic acid,chlorogenic acid,caffeic acid and rutin.The similarities of ten batches samples were lager than 0.9,and ten batches samples were classified into three groups by cluster analysis.Five major chemical components which closely related to the quality of material were selected by principal component analysis.Conclusion:The HPLC fingerprint and analytical model is stable,reliable and repeatable,which provides a reference for the quality evaluation and control of Blumea riparia.