干姜化学成分的分离鉴定

Separation and Identification of Chemical Constituents from Zingiberis Rhizoma

目的:研究干姜的化学成分。方法:利用Diaion HP-20、Toyopearl HW-40、ODS、硅胶柱层析及半制备液相色谱等多种色谱技术进行分离纯化,根据化合物的理化性质和光谱数据鉴定结构。结果:从干姜中分离并鉴定得到15个化合物,分别为:姜酮(1)、[6]-姜酚(2)、(E)-6-羟基-1-(4-羟基-3-甲氧基苯基)癸-4-烯-3-酮(3)、4-(4-羟基-3-甲氧基苯基)丁-3-烯-2-酮(4)、1-(4-羟基-3-甲氧基苯基)乙酮(5)、环-(色氨酸-缬氨酸)-二肽(6)、异落叶松脂素(7)、[4]-姜酚(8)、二氢阿魏酸乙酯(9)、阿魏酸乙酯(10)、松脂素(11)、8-羟基-1-(4-羟基-3-甲氧基)苯基-1-丙酮(12)、8-羟基-1-(4-羟基)苯基-1-丙酮(13)、4-(1-甲氧基乙基)-苯酚(14)、环(L-亮氨酸-L-脯胺酸)(15)。结论:其中,化合物5~7、9~15均为首次从该植物中分离得到。

Objective :To study the chemical constituents of Zingiberis Rhizoma.Methods:Diaion HP-20,Toyopearl HW-40,ODS, silica gel column and semi-preparation liquid were used to separate and purify the structure,and the structure was identified according to the physical and chemical properties,and spectral data of the compounds.Results :The structures of 15 compounds were isolated from Zingiberis Rhizoma,which were identified as zingerone(1),[6]-gingerol(2),(E)-6-hydroxy-1-(4-hydroxy-3-methoxyphenyl)dec-4-en-3-one(3),4-(4-hydroxy-3-methoxyphenyl)butan-3-en-2-one(4),1-(4-hydroxy-3-methoxyphenyl)ethanone(5),ring-(tryptophan- valine)-dipeptide(6),isolariciresinol(7),[4]-gingerol(8),ethyl-dihydroxyferulate(9),ethyl-ferulate(10),(+)-pinoresinol(11),8-hydroxy-1-(4-hydroxy-3-methoxy)phenyl-1-propanone(12),8-hydroxy-1-(4-hydroxy)phenyl-1-propanone(13),4-(1-methoxyethyl)-phenol(14),ring(L-leucine-L-proline)(15).Conclusion:Compounds 5-7,9-15are isolated from this plant for the first time.