摘要
目的:优选混合酶法提取五指毛桃多糖的工艺。方法:通过单因素试验设计,以多糖收率为指标,考察不同影响因素对工艺的影响,并根据单因素结果通过D-最优混料设计优化复合酶的配比,用Box-Behnken试验设计法对得出的影响因素进行筛选。结果:混合酶最佳比例为果胶酶占25.2%、纤维素酶占66.7%、甘露聚糖酶占8.1%;混合酶法提取五指毛桃多糖最优工艺为加酶量5.0%、溶媒倍数36倍、反应体系p H 4.7、酶反应温度42.5℃及酶反应时间294 min,五指毛桃多糖收率在1.39%左右。结论:利用Box-Behnken效应面法优选的五指毛桃多糖复合酶提取工艺稳定可行,可用于五指毛桃多糖的提取。
Objective:To optimize conditions of enzymatic extraction of Ficus simplicissima.Methods:Through the single factor experiment design,the effect of different factors on the process was investigated with the yield of polysaccharide as the index,optimization of compound enzyme ratio by D-optimal mixture design based on single factor results.Based on the response surface methodology by using Box-Behnken design,different influence factors were examined.Results:The optimal combination of enzymes were 25.2% pectinase,66.7% cellulase and 8.1% mannose.The dosage of the mixed enzyme was 5.0%,the solvent ratio was 36,initial reaction system pH was 4.7,enzymatic hydrolysis temperature was 42.5℃,and enzymatic hydrolysis time was 294 min.Under those conditions,the yield of polysaccharides of Ficus simplicissima was 1.39%.Conclusion:The Box-Behnken response surface methodology which used in the experiment to optimize the enzymatic extraction process for the polysaccharide of Ficus simplicissima is stable,effective and feasible.
作者
陈祝霞
刘伟锐
赵荣军
陈亮
钟鸣
CHEN Zhu-xia;LIU Wei-rui;ZHAO Rong-jun;CHEN Liang;ZHONG Ming(Dispensary of Taixing People's Hospital in Jiangsu Province,Taizhou 225400,China;Guangzhou Hanfang Pharmaceutical Corn- pang Litmited,Guangzhou 510240,China;National Engineering Research Center for Modernization of Extraction and Separation Process of TCM,Guangzhou 510970,China)
出处
《中药材》
CAS
北大核心
2018年第2期400-406,共7页
Journal of Chinese Medicinal Materials
基金
广州市科技计划项目(201503020011)
关键词
五指毛桃
多糖
混合酶法
BOX-BEHNKEN效应面法
Ficus simplicissima Lour.
Polysaccharides
Enzymatic extraction with mixed enzymes
Box-Behnken design and response surface methodology