信号转导子和转录激活子-1、干扰素调节因子-1及干扰素-γ在支气管哮喘儿童中的表达及其临床意义

Expression and clinical significance of signal transducer and activator of transcription-1,interferon regulatory factor-1,and interferon-γ in children with bronchial asthma

目的 在支气管哮喘儿童外周血单个核细胞中,检测信号转导子和转录激活子-1 (STAT1)、干扰素调节因子-1(IRF1)及干扰素-γ(IFN-γ)的表达,以进一步探讨其在哮喘病理生理机制中的作用.方法 选取2017年9月至2018 年5 月,我院儿科门诊就诊以及住院的急性发作期支气管哮喘患儿38例作为哮喘组,该38 例患儿经规范治疗后,哮喘良好控制患儿作为哮喘治疗有效组(n=23),哮喘部分控制及未控制患儿作为哮喘治疗无效组(n=15).选取同期健康儿童25例作为对照组.应用 RT-PCR 和 Western blot 方法 检测各组外周血单个核细胞中 STAT1、 IRF1 及 IFN-γ 的mRNA和蛋白表达,分析支气管哮喘儿童 STAT1、IRF1 及 IFN-γ 的 mRNA 和蛋白表达水平相关性.结果 哮喘组、哮喘治疗有效组、哮喘治疗无效组STAT1、IRF1 mRNA及蛋白表达均高于对照组,差异有统计学意义(P<0. 05);哮喘治疗有效组IRF1 mRNA及蛋白表达均低于哮喘治疗无效组,差异均有统计学意义(P<0. 05);哮喘组、哮喘治疗有效组、哮喘治疗无效组INF-γ mRNA及蛋白表达均低于对照组,差异有统计学意义(P<0. 05).结论 STAT1在支气管哮喘患儿中高表达,IFN-γ在支气管哮喘患儿中低表达. IRF1在支气管哮喘患儿中高表达,且其表达与治疗有效与否密切相关.

Objective To study the expression profiles of signal transducer and activator of transcription-1(STAT1),interferon regulatory factor-1(IRF1),and interferon-γ(IFN-γ)in peripheral blood mononuclear cells in children with bronchial asthma,in order to further study their roles in the pathogenesis of asthma.Methods Thirty-eight children with acute exacerbation of bronchial asthma who visited pediatric outpatient clinic or hospitalized in pediatric department from September 2017 to May 2018 were enrolled as the asthma group.After standardized treatment,the 38 children with asthma were divided into the effective asthma treatment group(n =23)and the ineffective asthma treatment group (n =15),according to the treatment effect.Twenty-five healthy children were enrolled as the control group.RT-PCR and Western blot were used to measure the mRNA and protein expression levels of STAT1,IRF1,and IFN-γ in peripheral blood mononuclear cells.The correlations between the mRNA and protein expression of STAT1,IRF1,and IFN-γ were analyzed.Results The asthma group,the effective asthma treatment group and the ineffective asthma treatment group had significantly higher mRNA and protein expression levels of STAT1 and IRF1 than those of the control group (P <0.05).The ineffective asthma treatment group had significantly higher mRNA and protein expression levels of IRF1 than those of the effective asthma treatment group (P <0.05).The asthma group,the effective asthma treatment group and the ineffective asthma treatment group had significantly lower mRNA and protein expression levels of INF-γ than those of the control group (P <0.05).Conclusion The expression level of STAT1 increases in children with asthma,while the expression level of IFN-γ decreases in those children.The expression level of IRF1 increases in children with asthma and is closely related to the treatment effectiveness.