飞蝗储存蛋白家族基因的表达与免疫功能分析

Expression and Immune Functional Analysis of Hexamerin Gene Family in Locusta migratoria( Acridoidea: Oedipodidae)

储存蛋白是一种重要的功能蛋白,在昆虫的生长发育和机体免疫应答反应中发挥重要作用。为了进一步分析飞蝗储存蛋白家族基因的免疫功能,通过qPCR对飞蝗储存蛋白家族基因的4个成员(L.m.Hx1、L.m.Hx2、L.m.Hx3和L.m.Hx4)在成虫不同组织的表达进行定量分析,并选取表达量较高的表皮和脂肪体为实验材料对飞蝗成虫进行大肠杆菌刺激实验。结果显示:菌刺激后,L.m.Hx1和L.m.Hx2的相对表达量均呈现先下降后上调的趋势,L.m.Hx3和L.m.Hx4则呈现先上调后下降的趋势,表明飞蝗储存蛋白家族基因均具有一定的免疫功能,L.m.Hx1和L.m.Hx2负向调控飞蝗的免疫过程,而L.m.Hx3和L.m.Hx4则正向调控飞蝗的免疫过程,因此家族成员之间可能存在相互补偿效应。为进一步探讨飞蝗储存蛋白家族基因的免疫功能、相互作用机制及制定害虫防治的策略提供参考。

Hexamerin is a key functional protein and plays an important role in the growth and development of insects and the immune response.To further analyze the immune function of the hexamerin gene family in Locusta migratoria, qPCR method was adopted to quantitatively analyze the expression of 4members (L.m.Hx1,L.m.Hx2,L.m.Hx3 and L.m.Hx4)of the hexamerin gene family in different tissues of the adult stages in this paper,and the cuticle and fat body with higher expression levels were selected as materials to conduct E.coli stimulation experiments on adult Locusta migratoria.The results showed that after the bacteria stimulated,the relative expression levels of L.m.Hxl and L.m.Hx2 showed trends from declining to rising,while the relative expression levels of L.m.Hx3 and L.m.Hx4 showed trends from rising to declining,which indicated that the genes of the hexamerins family had immune function. L.m.Hx1 and L.m.Hx2 showed negative regulation of immune response,L.m.Hx3 and L.m.Hx4 showed positive regulation of immune response,and hexamerin gene family members manifest functional compensation.Above results provided basis for further studying the immune function and mechanism of hexamerin gene family in Locusta migratoria,as well as reference for pest control.