土荆芥对幽门螺杆菌感染小鼠肠道菌群多样性的影响

Effect ofChenopodium ambrosioides L. on the diversity of intestinal flora in mice infected with Helicobacter pylori

目的观察幽门螺杆菌(HP)诱导小鼠胃炎模型肠道菌群结构和丰度的变化。获取盲肠内容物进行测序分析,通过分析肠道菌群结构的改变探究长期服用土荆芥提取物对小鼠的影响。方法雄性昆明小鼠30只,随机分为空白组10只、模型组10只,土荆芥组10只。幽门螺杆菌感染成模后给药4周,同一时间取各组小鼠的新鲜直肠粪便。在Illumina HiSeq PE250高通量测序平台上对粪便样本中细菌16S rDNA-V3-V4区进行测序,对肠道菌群结构、丰度进行定量分析。结果在门水平,幽门螺杆菌感染模型组拟杆菌门、变形菌门、疣微菌门、软壁菌门、放线菌门相对丰度降低,厚壁菌门相对丰度升高,与空白组和土荆芥组均有明显差异;在属水平,模型组乳杆菌属、双歧杆菌属相对丰度降低,拟杆菌属、瘤胃球菌属、颤杆菌和理研菌属、肠球菌属、肠单胞菌属等相对丰度升高。土荆芥组肠单胞菌属和理研菌属相对丰度升高,肠球菌属相对丰度降低。结论 HP的定植会显著改变肠道菌群结构,肠道菌群的丰度和多样性降低,HP感染胃炎的发病过程可能与益生菌群减少,有害菌群增加有关。土荆芥干预可以改善HP感染对肠道菌群的影响,增加菌群多样性。

Objective To observe the changes of intestinal flora structure and abundance in mice with gastritis induced by Helicobacter pylori (HP). The cecal contents were obtained for sequencing analysis,and the effects of long-term use of the extract of Chenopodium ambrosioides L. on mice were investigated by analyzing the changes in the structure of the intestinal flora. Methods Thirty male Kunming mice were randomly divided into blank group (n = 10),model group (n = 10) and CAL (Chenopodium ambrosioides L.)group (n = 10). After HP infection was established,they were administered drugs for 4 weeks,and fresh rectal stools of each group of mice were taken at the same time. The 16 S rDNA-V3-V4 region of the fecal samples was sequenced on an Illumina Hi Seq PE250 highthroughput sequencing platform to quantify the structure and abundance of the intestinal flora. Results At the level of the portal,the relative abundance of the Helicobacter pylori infection model group,such as Bacteroides, Proteobacteria, Mycobacterium, Softwalled bacteria,and actinomycetes,were decreased,and the relative abundance of the thick-walled bacteria increased in the model group. There were significant differences with the blank group and the schizonepeta group; at the genus level,the relative abundance of the model group Lactobacillus and Bifidobacterium decreased, Bacteroides, rumen cocci, dystrophic and Phytophthora,Enterococcus,increased. The relative abundance of the genus Monocytogenes was elevated. The relative abundance of Enterobacter and Limerella was increased,and the relative abundance of Enterococcus was decreased. Conclusion This study suggests that colonization of HP significantly could change the structure of the intestinal flora,and the abundance and diversity of the intestinal flora could be reduced. The pathogenesis of HP infection with gastritis may be related to the decrease of probiotics and the increase of harmful flora. Intervention of E. striata can improve the effect of HP infection on intestinal flora and increase the diversity of flora.