食管腺癌组织CEACAM1表达临床意义

Expression of CEACAM1 and its clinical significance in esophageal adenocarcinoma tissues

目的 癌胚抗原相关细胞黏附分子1(carcinoembryonic antigen related cell adhesion molecule 1,CEACAM1)与多种恶性肿瘤的发生发展密切相关.本研究探讨CEACAM1在食管腺癌组织及癌旁正常食管组织中表达及临床意义.方法 收集河北省中医院、河北省肿瘤医院、河北省人民医院、邯郸第一医院和磁县人民医院病理科标本库2004-01-01-2012-12-31临床资料完整、手术切除石蜡包埋的食管腺癌及癌旁正常食管组织标本各128例,采用免疫组织化学方法检测癌组织及正常食管组织中CEACAM1表达水平和表达方式,并分析CEACAM1表达与食管腺癌患者各项临床病理参数及预后关系.结果 CEACAM1在正常食管组织中基本无表达,在食管腺癌组织中高表达,差异有统计学意义,Z=13.36,P<0.001,且CEACAM1表达水平与患者肿瘤分化程度(Z=2.52,P=0.011)和病理分期(Z=2.19,P=0.028)有关联,而与患者年龄(Z=1.04,P=0.297)和性别(Z=0.06,P=0.949)无关联.在食管腺癌组织细胞中CEACAM1表达方式分为细胞膜表达、细胞质表达和二者均有的混合表达,食管腺癌组织病理分化程度越低、分期越晚,细胞质表达越多[高-中vs 低分化(χ2=8.649,P=0.013);Ⅰ~Ⅱvs Ⅲ~Ⅳ期(χ2=25.410,P<0.001)].进一步研究发现CEACAM1表达与患者5年生存率密切相关,表达水平高且以细胞质表达为主的患者5年生存率低于表达水平低(χ2=30.511,P<0.001)且以细胞质表达为主的患者(χ2=36.438,P<0.001).结论 CEACAM1在食管腺癌组织中表达水平明显增高,其表达与食管腺癌患者病理分化与分期及预后有关,表明CEACAM1可能是食管癌的新靶点.

OBJECTIVE CEACAM1 is closely related to the occurrence and development of a variety of malignant tumors.This study investigated the expression and clinical significance of CEACAM1in esophageal adenocarcinoma and adjacent normal esophageal tissues.METHODS Collecting the pathological specimens of Hebei Provincial Hospital of Traditional Chinese Medicine,Hebei Provincial Cancer Hospital,Hebei Provincial People's Hospital,First Hospital of Si- chuan Province,People's Hospital of Cixian County,and other clinical data from 2004-01-01 to 2012-12-31,surgically removed paraffin-embedded,the esophageal adenocarcinoma and adjacent normal esophageal tissue specimens were 128cases.The expression level and expression pattern of CEACAM1in cancer tissues and normal esophageal tissues were detected by immunohistochemistry.The correlation between CEACAM1expression and clinical pathological parameters andprognosis of patients with esophageal adenocarcinoma was analyzed.RESULTS The CEACAM1was not expressed innormal esophageal tissues;it was expressed in esophageal adenocarcinoma tissues,significantly,Z=13.36,P<0.001.CEACAM1's expression was correlated with the tumor differentiation(Z=2.52,P=0.011)and clinical stages(Z=2.19,P=0.028)at the significant level,whereas it had no obvious correlation with age(Z=1.04,P=0.297)or gender(Z=0.06,P=0.949).The expression of CEACAM1in esophageal adenocarcinoma cells was divided into cell membrane expression(33cases),cytoplasm expression(58cases)and the mixed expression(31cases)of both.More cytoplasm was expressed in esophageal adenocarcinoma tissue in late clinic stage with low degree of pathological differentiation(High-middle vs low degree(χ2=8.649,P=0.013);stageⅠ-Ⅱvs Ⅲ-Ⅳ(χ2=25.410,P<0.001).The patients with high expression level and cytoplasm expression of CEACAM1had significantly lower five-year survival rate than that of the patients with low expression level(χ2=30.511,P<0.001)and other expression patterns(χ2=36.438,P<0.001).CONCLUSIONS The expression of CEACAM1in esophageal adenocarcinoma is significantly higher.Its expression affects thepathological differentiation and staging of the patients and closely related to the prognosis of the patients.It is suggestingthat CEACAM1may be a new target of esophageal cancer.