基于分子印迹柱的在线速测装置用于菊酯类农药代谢产物快速检测

Molecularly imprinted column based online analysis device for the rapid detection of pyrethroid metabolites

本研究将光电检测器与分子印迹柱联用,搭建了菊酯类农药代谢产物在线速测装置,样液预先在印迹柱中吸附和洗脱,并采用硝基苯肼显色法对洗脱液中农药代谢产物进行定量检测。以菊酯类农药代谢产物间苯氧基苯甲醛为研究对象,得到最优检测条件为:吸附阶段样液分12段吸附,每段吸附1 min;洗脱阶段采用0. 4 mL洗脱剂在印迹柱内震荡洗脱1 min;检测阶段洗脱液与显色剂在50℃控温条件下,反应6 min。在最优的实验条件下,该速测装置的检出限为1. 13μg/mL,且在19 min内便能完成对样品的检测。采用该装置对样品中间苯氧基苯甲醛进行加标回收实验,回收率为72. 4%~90. 3%。

A rapid analysis device was developed for the detection of pyrethroid metabolites by combining photoelectric detector and molecularly imprinted column. The detection process consisted of two steps including sample absorption-desorption and colorimetric detection of target in presence of nitrophenylhydrazine. 3-Phenoxybenzaldehyde,one type of pyrethroid metabolite,was chosen as target to investigate the performance of device under various parameters. The results indicated that the sample solution was divided into twelve sections for the absorption of target,and each section was maintained in the molecularly imprinted column for 1 min. After absorption,0. 4 m L elution buffer was used for the desorption of target from molecularly imprinted column in 1 min. For the colorimetric detection,the chromogenic reagent was added to elution buffer,and then incubated for 6 min at 50℃. Under the optimized conditions,the detection process was finished in 19 min and the limit of detection was 1. 13μg/m L. Additionally,the recoveries ranged from 72. 4% to 90. 3% were achieved in the spiked real samples. These results demonstrated that this device was appropriate for 3-phenoxybenzaldehyde analysis in water samples.