神经肽S受体1和消极应对在慢性心理应激反应中的中介效应

The mediating effects of neuropeptide S receptor 1 and negative coping on chronic psychological stress

目的探讨神经肽S受体1(neuropeptide S receptor 1,NPSR1)和消极应对在慢性心理应激反应中的中介效应。方法采用分层随机整群抽样方法在广西南丹县抽取366名当地普通居民对其进行生活事件量表(the life eventss cale,LES)、简化症状自评量表、简易应对方式量表(the simplified coping style questionnaire,SCSQ)的测试,采用酶联免疫吸附测定检测调查对象血浆中的NPSR1表达水平,利用SPSS21.0和AMOS21.0统计学软件进行统计分析。结果慢性心理应激水平为(25.24±29.26)分,消极应对(14.84±4.35)分,躯体因子、焦虑因子、抑郁因子、敌对因子分别为(1.58±0.58)分,(1.43±0.56)分,(1.44±0.55)分,(1.23±0.37)分。生活事件总分与消极应对、躯体因子、焦虑因子、抑郁因子、敌对因子呈正相关(r=0.23~0.46,P<0.05),与NPSR1水平呈负相关(r=-0.26,P<0.05);NPSR1水平与躯体因子、焦虑因子、抑郁因子、敌对因子呈负相关(r=-0.18^-0.61,P<0.05);消极应对与躯体因子、焦虑因子、抑郁因子、敌对因子呈正相关(r=0.12~0.27,P<0.05)。在结构方程的中介模型中,拟合指标均达到理想值(χ2=0.349,GFI=0.993,AGFI=0.979,RMSEA=0.018,CFI=0.998),神经肽S受体1与消极应对对慢性心理应激引起的情绪反应的中介效应均显著(P<0.05),效应值分别为-0.08,0.05。根据Bootstrapping法检验中介模型的结果显示:Bias-Corrected的标准化总效应、标准化间接效应、标准化直接效应的95%CI均不与0相交。结论NPSR1和消极应对在由应激引起的应激性情绪反应中起到双重中介效应。

Objective To explore the mediating effects of neuropeptide S receptor 1 and negative coping in chronic stress induced emotional responses.Methods A survey of 366 local residents who were chosen from County of Nandan in Guangxi Province by stratified random sampling method.Questionnaires of the life events scale(LES),the simplified symptom checklist and the Simplified Coping Style Questionnaire(SCSQ)were used.The level of NPSR1 was measured by Elisa.Data was analyzed by SPSS 21 and AMOS 21 statistical software for statistical analysis.Results The level of chronic psychological stress was(25.24±29.26)and score of negative coping was(14.84±4.35).Somatic factor,anxiety factor,depressive factor and hostile factor were(1.58±0.58),(1.43±0.56),(1.44±0.55),(1.23±0.37).The total score of life events was positively correlated with negative coping,somatic factor score,anxiety factor score,depression factor score(r=0.23-0.46,P<0.05),while negatively correlated with the expression level of detection of NPSR1 in plasma(r=-0.26,P<0.05).NPSR1 level was negatively correlated with body factor score,anxiety factor score,depression factor score and hostility factor score(r=-0.18--0.61,P<0.05).Negative coping was positively correlated with body factor score,anxiety factor score,depression factor score and hostility factor score(r=0.12- 0.27,P<0.05).Both neuropeptide S receptor 1 and negative response had significant mediating effects on emotional responses induced by chronic psychological stress(P<0.05),and the effect values were -0.08 and 0.05,respectively.According to the Bootstrapping method,the results of the mediation model showed that Bias-Corrected of 95%CI of the total standardization effect,the indirect standardization effect,the direct standardization effect were not including 0.Conclusion NPSR1 and negative coping plays a dual mediating effect on stress induced emotional responses.