长链非编码RNA RPL41影响肾癌细胞迁移和侵袭过程

Long non-coding RNA RPL41 affects renal cell carcinoma migration and invasion

目的观察长链非编码RNA(lncRNA)RPL41对肾癌细胞迁移和侵袭过程的影响。方法实时荧光定量聚合酶链反应(FQ-PCR)检测26例肾癌组织及3种肾癌细胞株(786-0、769-P、ACHN)中lncRNARPL41的表达。通过慢病毒筛选稳转株后,噻唑蓝(MTT)实验、Transwell检测lncRNARPL41对肾癌细胞的潜在生物功能影响。结果lncRNARPL41在26例肾癌组织中的表达量显著低于其在正常组织中的表达量(40.69±0.74比82.92±1.26,P=0.000,n=26),且lncRNARPL41在肾癌细胞株769-P中表达水平最高,其次是786-0,在ACHN中的表达量最低(F=65.810,P=0.000,n=5)。细胞功能实验表明,lncRNARPL41对肾癌细胞迁移和侵袭过程产生了显著影响。786-0组迁移实验中细胞穿透数目分别为(150.90±14.89)个比(247.30±13.17)个比(260.30±18.96)个(n=10,P=0.000);ACHN组迁移实验中细胞穿透数目分别为(130.90±14.89)比(240.30±18.96)比(227.30±13.17)个(n=10,P=0.000)。786-0组侵袭实验中细胞穿透数目分别为(72.90±14.89)个比(94.30±13.17)个比(102.20±16.19)个(n=10,P=0.008);ACHN组侵袭实验中细胞穿透数目分别为(72.90±14.89)个比(97.30±18.96)个比(84.30±13.17)个(n=5,P=0.007)。结论lncRNARPL41在肾癌组织及细胞株中明显低表达,同时对肾癌细胞株的迁移和侵袭功能产生了明确的影响。

Objective To observe the effect of long non-coding RNA (lncRNA) RPL41 on the migration and invasion of renal cancer cells. Methods Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect the expression of lncRNA RPL41 in 26 renal cell carcinoma and 3 renal cell carcinoma cell lines (786-0, 769-P, ACHN). After screening for stable transfectants by lentivirus, methyl thiazol tetrazolium (MTT) assay and Transwell were used to detect the potential biofunctional effects of long-chain non-coding RNA RPL41 on renal cancer cells. Results The expression of lncRNA RPL41 in 26 cases of renal cell carcinoma was significantly lower than that in normal tissues (40.69±0.74 vs. 82.92±1.26, n=26, P=0.000), and lncRNA RPL41 had the highest expression level in renal cancer cell line 769-P, followed by 786-0, and the lowest expression in ACHN (F=65.810, P=0.000, n=5). Cell function experiments showed that lncRNA RPL41 had a significant effect on the migration and invasion of renal cancer cells. The number of cell penetration in the migration test of 786-0 group was (150.90±14.89) cells vs. (247.30±13.17) cells vs. (260.30±18.96) cells (n=10, P=0.000); the cell penetration number in the ACHN group migration experiment was (130.90±14.89) cells vs. (240.30±18.96) cells vs. (227.30±13.17) cells (n=10, P=0.000). The number of cell penetration in the invasion test of 786-0 group was (72.90±14.89) cells vs. (94.30±13.17) cells vs. (102.20±16.19) cells (n=10, P=0.008); the number of cell penetration in the ACHN group was (72.90±14.89) cells vs. (97.30±18.96) cells vs. (84.30±13.17) cells (n=5, P=0.007). Conclusion lncRNA RPL41 is significantly under-expressed in renal cell carcinoma tissues, and has a clear influence on the migration and invasion functions of renal cancer cell lines.