丹参细胞分裂素调控因子序列特征分析

Analysis of Sequence Characteristics of Cytokinin Response Regulator in Salvia miltiorrhiza

细胞分裂素通过双元信号系统感知外界环境刺激并作出应答。其中细胞分裂素响应调控因子(responseregulator,RR)是该双元信号系统中的重要构成。采用生物信息学的方法,对丹参(Salvia miltiorrhiza)细胞分裂素调控因子进行序列特征和组织表达特性分析。结果发现,丹参基因组中共有15个丹参细胞分裂素调控因子SmRR,序列比对与系统发生分析表明,SmRR1～SmRR7与拟南芥A类RR亲缘关系比较接近,划分为A类RR,SmRR8～SmRR15与拟南芥B类RR亲缘关系近,归为B类;在A类SmRR中,REC接受域含有保守的DDK残基,因此这些基因被推测有完整的功能,B类SmRR具有高度保守Myb_SHAQKYF结构域。两类SmRR的内含子、外显子组成也具有相对的保守性,顺式调控元件的分析发现,SmRR存在很多胁迫和生长调节物质的响应元件。表达量热图显示A类和B类SmRR基因分别在叶和根中表达量较高。

Cytokinin plays an important role in the growth and development of plants,which responds to external environmental stimuli through the two-component system in plant. The response regulators (RRs)are critical members in the two-component system. Through genome-wide identification,15 putative SmRR genes were identified from the genome of Salvia miltiorrhiza. Based on conserved domains and phylogenetic analysis,15 SmRR genes were divided into two subfamilies,type-A (SmRR1–SmRR7) and type-B (SmRR8–SmRR15) . Sequence analysis of the SmRR gene family in S. miltiorrhiza,including gene structure,sequence features,cis-acting element,conserved motifs,and expression in different organs, were carried out. The results showed that the REC domain contains conserved DDK amino-acid residues in type-A SmRR,type-B SmRR has conserved Myb-like domain. The exon and intron number of SmRR also has relative conservatism. The analysis of cis-regulatory elements revealed that there are many stress and growth regulating substance response elements in SmRR. Differential transcript abundance was detected for SmRRs in different organs and tissues.