黄芪、莪术联合顺铂对人肝癌裸鼠原位移植瘤内微血管的影响

Effect on Microvascular of Human Hepatocarcinoma Transplantation Model Nude Mice by Compatibility of Radix Astragali and Curcuma and Combination with Cisplatin

目的:探讨黄芪、莪术配伍联合顺铂(DDP)抗肝癌新生血管生成的作用机制。方法:构建人肝癌裸鼠原位移植瘤模型。成瘤后,将其随机分为8组:模型对照组、阳性对照组(DDP)、黄芪莪术配伍高剂量组(H)、黄芪莪术配伍中剂量组(M)、黄芪莪术配伍低剂量组(L)、黄芪莪术配伍高剂量+顺铂组(H+DDP)、黄芪莪术配伍中剂量+顺铂组(M+DDP)、黄芪莪术配伍低剂量+顺铂组(L+DDP),每组8只,给药15d,检测肿瘤新生微血管密度计数(CD34)、MMP-2、bFGF。结果:与模型对照组相比,H、M、H+DDP、M+DDP组移植瘤组织微血管数目均减少(P<0.05或P<0.01),血清中bFGF、MMP-2含量均明显降低(P<0.01)。与DDP组相比,H、M、H+DDP、M+DDP组移植瘤组织微血管数目均减少(P<0.05或P<0.01),H、H+DDP组血清中bFGF、MMP-2含量均明显降低(P<0.01)。结论:黄芪、莪术配伍可能是通过下调MMP-2、bFGF的表达对新生血管生成产生抑制作用。

Objective: To study the mechanisms of radix astragali and curcuma and combination with cisplatin(DDP) on angiogenesis of hepatocellular carcinoma. Method: Human hepatocarcinoma cells HepG2 were transplanted into nude mice to form the study model, and the model mice were divided at random into 8 groups: model group, cisplatin(DDP) group, high dosage of radix astragali and curcuma(H), intermediate dosage of radix astragali and curcuma(M), low dosage of radix astragali and curcuma(L), high dosage and cisplatin group(H+DDP), intermediate dosage and cisplatin group(M+DDP), low dosage and cisplatin group(L+DDP), 8 in each group. The level of tumor microvessel density(CD34), matrix metalloproteinase-2 and basic fibroblast growth factor were detected. Result: Compared with the model group, the level of tumor microvessel density(CD34) was significantly lower(P<0.05,P<0.01), and the contents of bFGF and MMP-2 in blood serum were obviously decreased(P<0.01) in H, H+DDP, M+DDP and L+DDP groups. Compared with the DDP group, the level of tumor microvessel density(CD34) was significantly lower(P<0.05,P<0.01) in H, H+DDP, M+DDP and L+DDP groups, and the contents of bFGF and MMP-2 in blood serum were obviously decreased(P<0.01) in H and H+DDP groups. Conclusion: Compatibility of radix astragali and curcuma may be to inhibit the angiogenesis by downregulating the expressions of MMP-2 and bFGF.