左归丸对去卵巢大鼠骨组织降钙素受体蛋白表达的影响

Effect of Zuogui Pills on Calcitonin Receptor Protein Expression of Bone Tissue in Ovariectomized Rats

目的:探究左归丸对去卵巢大鼠骨组织中降钙素受体蛋白(CTR)表达的影响。方法:制备去卵巢大鼠模型,实验分为假手术组、去卵巢组、左归丸组。采用ELISA法检测血清中ALP和TRAP含量,免疫组织化学染色法检测股骨组织ALP蛋白表达,Western Blot法检测股骨组织CTR蛋白表达情况。结果:与假手术组比较,去卵巢组血清中ALP和TRAP含量明显升高(P<0.01),股骨组织ALP和CTR蛋白表达明显下降;与去卵巢组比较,左归丸组血清中ALP和TRAP明显降低(P<0.05),股骨组织ALP和CTR蛋白表达明显升高。结论:左归丸能明显降低血清中ALP和TRAP的含量,显著升高ALP和CTR蛋白表达的水平,从而有效防治绝经后骨质疏松症。

Objective:To investigate the effect of Zuogui Pill on calcitonin receptor(CTR) protein expression in bone tissue of ovariectomized rats.Methods:Ovariectomized rats were randomly divided into three groups: shamgroup, ovariectomized group and Zuogui Pill group. The ALP and TRAP in serum were measured by ELISA method. The expressions of ALP and CTR protein of femur in rats were detected by immunohistochemical method and Western blot. Results:Compared withsham group, the ALP and TRAP in serum was significantly increased(P<0.01), and the expression of ALP and CTR protein was significantly decreased in ovariectomized rats. Compared with ovariectomized group, the serum ALP and TRAP were significantly decreased(P<0.05), and the expressions of ALP and CTR protein of femur in rat were significantly increased in Zuogui Pill group.Conclusion:Zuogui Pill can significantly reduce the serum levels of ALP and TRAP, and improve the levels of ALP and CTR protein expressionso as to effectively treat postmenopausal osteoporosis.