番茄红素对人体血清自由基的清除作用

Scavenging Effect of Lycopene on Free Radical of Human Serum

评价番茄红素对人体血清自由基的清除效果。志愿者在服用不同剂量(5、10、15 mg/d)的番茄红素后在不同时间(15、30、45、60 d)下考察其血样中总抗氧化能力(T-AOC)、超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)和丙二醛(MDA)的含量变化,同时考察第60 d时各组志愿者在补充番茄红素次日进行力竭性运动后,其运动前后血样中T-AOC,SOD,GSH-PX和MDA含量变化。实验结果表明,相比空白对照组而言,不同剂量组的受试者在服用番茄红素后,随着服用天数和服用剂量的增加,其体内血清中的GSH-PX酶活力和SOD酶活力呈现升高趋势,但GSH-PX的酶活力只有在低剂量组摄入天数为15 d时表现出差异显著,其余随天数和剂量变化均不显著。SOD的酶活力只有在低、中剂量组摄入天数为30 d时表现出差异显著,其余随天数和剂量变化均不显著。而T-AOC酶活力呈现升高趋势且差异不显著,MDA含量呈现下降趋势且差异不显著。而且,相比运动之前,经过力竭性运动后,不同剂量组中的T-AOC、SOD含量和GSH-PX含量均有显著降低,而MDA含量则有显著升高。提前摄入番茄红素胶囊,能够减少体内抗氧化酶的消耗和体内的MDA的含量。综上所述,番茄红素能够有效清除人体血清中的自由基。

In this study,the scavenging effect of lycopene on free radical of human serum was investigated. Volunteers were taking different doses (5,10 and 15 mg/d) of lycopene,respectively.After adding the lycopene in 15,30,45,and 60 d,the total antioxidant capacity in the blood (T-AOC) and the content of superoxide disproportionation enzyme (SOD),glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured. Moreover,after adding the lycopene in 60 days,all the participants would do inexhaustible movement in next day to measure the changes of T-AOC,SOD,GSH-PX and MDA content in before and after the movement.Results showed that compared with the blank control group,after volunteers taking lycopene,with the increase of times and doses,enzyme activity of GSH-PX and SOD tend to rise,and the enzyme activity of GSH-PX showed significant difference only when the intake days of the low-dose group were 15 d,and the enzyme activity of SOD showed significant difference only when the intake days of low and medium dose groups were 30 d,and the rest showed no significant change with days and dosage increasing.The T-AOC tend to rise and no significant change was observed with the days and dosage increasing.The MDA content showed decline and no significant change was observed with the days and dosage increasing.Moreover,after the strong exercise,the content of T-AOC,SOD and GSH-PX in different dosage groups decreased significantly,while the MDA content increased significantly. Taking lycopene capsules can reduce the consumption of antioxidant enzymes and MDA content in vivo.In conclusion,lycopene could effectively eliminate free radicals in human serum.