摘要
利用在线分析软件对嗜铁钩端螺旋菌中9种与铁硫簇结构、功能及生物合成相关蛋白质基本性质、活性位点、结构和蛋白相互作用网络等方面进行了预测。9种蛋白质包含7种酶和2种非酶蛋白,酶类蛋白与机体蛋白质裂解,翻译、信号转导和能量代谢相关,非酶类蛋白为生长因子和转运结合蛋白。所有蛋白的稳定性和等电点均有差异。WP_014960240.1、WP_014960241.1、WP_014960239.1和WP_014960235.1在ISC和NIF系统中发挥着重要的作用,参与铁硫簇的组装;WP_014959988.1可能主要参与细胞生长;WP_014960813.1和WP_014961639.1可能在能量代谢中发挥重要的作用;WP_014961659.1与铜金属解毒机制有关;WP_014962089.1参与亚硝酸盐的还原。该研究为进一步用分子生物学方法验证其相互作用机制提供给了理论依据,同时为菌株改良和生物浸矿奠定理论基础。
In L. f erriphilum 9 related-iron sulfur cluster proteins that structure, function and basic properties, biosynthesis related active sites of protein and protein interaction network etc were predicted by online analysis software. The 9 proteins contain 7 enzymes and 2 non enzymatic proteins. The enzyme proteins are involved in protein cleavage, translation, signal transduction and energy metabolism, and non enzyme proteins are growth factors and transport binding proteins. The stability and isoelectric point were different. WP_014960240.1, WP_014960241.1, WP_014960239.1 and WP_014960235.1 play important roles in ISC and NIF system, participating in the assembly of iron sulfur cluster; WP_014959988.1 may be involved in cell growth; WP_014960813.1 and WP_014961639.1 play important roles in energy metabolism; WP_014961659.1 may take part in the copper metal detoxification mechanism; WP_014962089.1 is possibly acting in the nitrite reduction. This study provides a theoretical basis for further validation of the interaction mechanism with molecular biology methods, and lays a theoretical foundation for the improvement of strains and bioleaching.
作者
冷非凡
罗文
李渊利
孙尚琛
王永刚
Leng Feifan;Luo Wen;Li Yuanli;Sun Shangchen;Wang Yonggang(School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou,730050;Lanzhou Lanshi Equipment Engineering Research Institute Co. Ltd, Lanzhou,730000)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2018年第12期5296-5303,共8页
Genomics and Applied Biology
基金
国家自然科学基金项目(No.31460032)资助
关键词
嗜铁钩端螺旋菌
铁硫簇蛋白
生物信息学分析
结构与功能
Leptospirillum ferriphilum
Iron sulfur cluster protein
Bioinformatics analysis
Structure and function