摘要
目的观察三七总皂苷(PNS)对急性心肌梗死(AMI)大鼠心功能的影响并探讨其对骨髓来源的间充质干细胞(BM-MSC)的动员作用。方法将48只大鼠随机分成假手术组、AMI组(模型组)、PNS低剂量组(在造模后给予100 mg/kg PNS灌胃)、PNS高剂量组(在造模后给予500 mg/kg PNS灌胃)。使用冠脉结扎法建立AMI动物模型,分别用高低剂量PNS处理7 d和21 d后,每组处死6只大鼠;处死前超声检测大鼠心功能,随后取外周血和心脏组织。流式细胞术检测CD90、CD105、CD54、CD106的频数反映BM-MSC的动员情况,ELISA检测干细胞因子(SCF)含量,2,3,5-三苯基氯化四氮唑(TTC)染色观察心肌梗死面积,原位末端转移酶标记技术(TUNEL)检测心肌细胞凋亡,免疫组织化学染色法检测CD105表达。结果与假手术组相比,模型组细胞凋亡水平显著增加; PNS处理7 d和21 d后,与模型组比较,低剂量和高剂量PNS组心肌梗死面积和细胞凋亡水平均降低。与假手术组比较,术后7 d和21 d,模型组左室射血分数(LVEF)和左室缩短分数(LVFS)降低,而收缩期左室内径(LVID)、舒张期左室内径(LVIDd)、左心室舒张末期容积(LVEDV)和左心室收缩末期容积(LVESV)增加;而PNS处理可有效改善上述指标。与模型组比较,PNS呈浓度依赖性增加外周血中CD90、CD105阳性细胞数和SCF的含量,降低CD54和CD106阳性细胞数。PNS处理组心脏组织内CD105表达明显高于模型组。结论 PNS处理可改善心肌梗死后左心室功能,可能与PNS抑制心肌细胞凋亡,促进BM-MSC动员有关。
Objective To observe the effect of panax notoginsenosides (PNS)on cardiac function of rats after acute myocardial infarction (AMI)and investigate the influence of PNS on the mobilization of bone marrow-derived mesenchymal stem cells (BM-MSCs).Methods A total of 48 rats were randomly assigned into the sham group,AMI group,low-dose PNS group [100mg/(kg·d)] and high-dose PNS group [500mg/(kg·d)].The rat model of AMI was established by coronary ligation,and 6rats were sacrificed in each group after 7 and 21 days of treatment with high and low doses of PNS. The heart function of rats was detected by echocardiography before execution,and peripheral blood and heart tissue were collected.Flow cytometry was used to test the proportions of CD90,CD105,CD54 or CD106 positive cells in the peripheral blood.ELISA was performed to measure the levels of stem cell factor (SCF) in the peripheral blood.I-FC staining was applied to evaluate the infarct size of myocardial tissues.TUNEL assay was carded out to determine the apoptosis of myocardial tissues and immunohistochemistry to determine the expression levels of CD105 in the infarction area.Results Compared with the sham group,the percentage of apoptotic cells in the AMI group significantly increased.Seven days or 21 days after the intervention with PNS,the infarction area and the apoptotic rates in the PNS treated groups were observably alleviated when compared with the AMI group.In addition,at 7 days and 21 days after operation,the LVEF and LVFS decreased, whereas the LVIDs,LVIDd,LVEDV and LVESV significantly decreased in the AMI groups when compared with the sham group.Treatment with PNS could effectively improve the above alterations.When compared with the AMI group,PNS treatment significantly increased the proportions of CD90 or CD105 positive cells and the concentration of SCF,whereas decrease the proportions of CD54 or CD106 positive cells in the peripheral blood in a dose-dependent manner.Moreover,the level of CD105 in the marginal zone of AMI was significant higher in the PNS treated groups when compared with that in the AMI group.Conclusion PNS treatment improves left ventricular function after AMI,which may be related to PNS inhibiting the apoptosis of myocardocytes and promoting the mobilization of BM-MSCs.
作者
周剑
吴荣
王龙
ZHOU Jian;WU Rong;WANG Long(Department of Cardiology,Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730000;Department of Geriatrics,First Hospital Affiliated to Lanzhou University,Lanzhou 730000,China)
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2018年第7期605-611,共7页
Chinese Journal of Cellular and Molecular Immunology
基金
甘肃省自然科学基金(148RJZA071)