摘要
目的:研究清肠温中方对葡聚糖硫酸钠(DSS)诱导溃疡性结肠炎(UC)大鼠肠黏膜屏障的影响。方法:采用4.5%DSS溶液制备大鼠UC模型,给予清肠温中方及美沙拉嗪灌胃,Western blot、Real time-PCR检测结肠claudin-1、claudin-4的表达;应用免疫组织化学法检测NF-κB p65蛋白的表达及分布。结果:与模型组相比,清肠温中方和美沙拉嗪组大鼠结肠claudin-1、claudin-4的基因和蛋白表达水平均显著升高(P <0.05),NF-κB p65平均光密度显著降低(P <0.05)。结论:清肠温中方可能通过下调NF-κB p65的表达,促进claudin-l、claudin-4的表达,修复肠粘膜损伤,最终达到治疗UC的目的。
Objective:The purpose of this paper is to study the effect of Qingchang Wenzhong Decoction on intestinal mucosal barrier in the rats with DSS-induced ulcerative colitis (UC)Methods:the UC model was made using 4.5%(w/v) DSS liquor.Meanwhile,the garage of Qingchang Wenzhong decoction and mesalazine was given.Claudin-1 and claudin-4 expression were detected by Western blot and Real time-PCR.The expression and distribution of NF-κB p65 protein were detected by immunohistochemistry.Resuhs:compared with the model group,the genes and protein levels of claudin-1 and claudin-4 of colitis rats with Qingchang Wenzhong decoction and mesalazine group significantly increased (P<0.05),and the average optical density of NF-κB p65 significantly decreased (P <0.05)Conclusion:Qingchang Wenzhong decoction could promote the expression claudin-1 and claudin-4 by reducing the expression of NF-κB p65,repairing intestinal mucosal damage and finally treating UC.
作者
毛堂友
裴文静
史瑞
赵兴杰
王志斌
陈晓伟
陈晨
石磊
谭祥
孙中美
丁庞华
李军祥
Mao Tangyou;Pei Wenjing;Shi Rui;Zhao Xingjie;Wang Zhibin;Chen Xiaowei;Chen Chen;Shi Lei;Tan Xiang;Sun Zhongmei;Ding Panghua;Li Junxiang(Gastroenterology Department,Dongfang Hospital,Beifing University of Chinese Medicine,No.6,1st section,Fangxingyuan,Fangzhuang,Beijing 100078,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2018年第7期1232-1237,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
北京中医药大学青年教师项目(2018-JYBZZ-JS097):基于肠道菌群调控肠上皮细胞TLR4/Blimp-1/NLRP12功能轴研究清肠温中方重建溃疡性结肠炎“肠道微生态-宿主免疫”平衡的机制研究,负责人:毛堂友
国家自然科学基金委青年基金项目(81403369):清肠温中方对溃疡性结肠炎大鼠MSP-RON通路的调控机制研究,负责人:史瑞
北京市科学技术委员会科技计划项目(Z151100003815011):十病十药研发--清肠温中方治疗溃疡性结肠炎的中药制剂的开发研究,负责人:李军祥.