清肠温中方促进DSS诱导溃疡性结肠炎大鼠结肠claudin-l、claudin-4表达修复肠黏膜屏障的机制研究

Study on the Mechanism of Qingchang Wenzhong Decoction in the Treatment of DSS-induced Ulcerative Colitis by Repairing Intestinal Mucosal Barrier Via Promoting the Expression of Claudin-l and Claudin-4

目的:研究清肠温中方对葡聚糖硫酸钠(DSS)诱导溃疡性结肠炎(UC)大鼠肠黏膜屏障的影响。方法:采用4.5%DSS溶液制备大鼠UC模型,给予清肠温中方及美沙拉嗪灌胃,Western blot、Real time-PCR检测结肠claudin-1、claudin-4的表达;应用免疫组织化学法检测NF-κB p65蛋白的表达及分布。结果:与模型组相比,清肠温中方和美沙拉嗪组大鼠结肠claudin-1、claudin-4的基因和蛋白表达水平均显著升高(P <0.05),NF-κB p65平均光密度显著降低(P <0.05)。结论:清肠温中方可能通过下调NF-κB p65的表达,促进claudin-l、claudin-4的表达,修复肠粘膜损伤,最终达到治疗UC的目的。

Objective:The purpose of this paper is to study the effect of Qingchang Wenzhong Decoction on intestinal mucosal barrier in the rats with DSS-induced ulcerative colitis (UC)Methods:the UC model was made using 4.5%(w/v) DSS liquor.Meanwhile,the garage of Qingchang Wenzhong decoction and mesalazine was given.Claudin-1 and claudin-4 expression were detected by Western blot and Real time-PCR.The expression and distribution of NF-κB p65 protein were detected by immunohistochemistry.Resuhs:compared with the model group,the genes and protein levels of claudin-1 and claudin-4 of colitis rats with Qingchang Wenzhong decoction and mesalazine group significantly increased (P<0.05),and the average optical density of NF-κB p65 significantly decreased (P <0.05)Conclusion:Qingchang Wenzhong decoction could promote the expression claudin-1 and claudin-4 by reducing the expression of NF-κB p65,repairing intestinal mucosal damage and finally treating UC.