青海省羊种布鲁菌地理分布特点

The geographical distribution characteristics of Brucella melitensis in Qinghai Province

目的对青海省已分离的布鲁菌菌株进行分种分型和遗传分析,了解青海省现有布鲁菌的地理分布特点、遗传类别及区域分布特征。方法采用多位点可变数目串联重复序列(MLVA)检测技术,对青海省已分离的布鲁菌株进行分子生物学分型。分型结果通过地理信息系统(GIS)进行地理分布特征描述。结果青海省的65株布鲁菌有羊种、牛种和猪种3个生物种,其中羊种布鲁菌有60株,是青海的优势种。MLVA的基因型有42、43、47、28、36、112和6型。地理分布特征显示42型分属A和B进化支,分布较广,43型的C进化支和47型的E进化支主要在青藏高原腹地出现。在Brucella2012 ML、慢数据库中搜索时,本研究中得到的基因型没有任何一种与数据库中的完全一致。结论青藏高原地区布鲁菌的MLVA基因型多样,地理分布较广;与其他地区的完全不一样,且不同的地方有不同的遗传变异。

Objective Molecular biological methods were used to classify and analyze the isolated Brucella strains,and to understand the geographical distribution characteristics,genetic types and regional distribution characteristics of Brucella in Qinghai Province.Methods Molecular biology typing of species of isolated Brucella strains in Qinghai was studied using Multiple Locus Variable-number tandem repeat Analysis (MLVA)technology. The classification results were described by geographical information system (GIS).Results There were 3 species BruceUa melitensis,Brucella abortus,and Brucella suis among the 65 strains of BruceUa in Qinghai.Brucella melitensis was the dominant species.The genotypes of MLVA were 42,43,47,28,36,112 and 6.The geographical distribution features showed that the 42 belonged to the evolutionary branches of A and B,which was widely distributed.The 43 of the C evolutionary branch and 47of the E evolution branch were mainly in the hinterland of the Qinghai-Tibet Plateau.When searching in the Brucella2012 MLVA database,none of the genotypes obtained in this study were identical to those in the database.Conclusions The MLVA genotypes of BruceUa are varied in Qinghai Tibet Plateau.They are widely distributed,completely different from those in other areas,and different genetic variations are found in different places.