间接血球凝集试验在内蒙古长爪沙鼠疫源地的应用与评价

Application and evaluation of indirect hemagglutination test in the Meriones unguiculatus natural focus of Inner Mongolia

目的分析并评价间接血球凝集试验(IHA)在内蒙古长爪沙鼠疫源地的应用。方法收集1996-2016年内蒙古各年度鼠疫监测总结和疫情分析报告(来自内蒙古综合疾病预防控制中心),分析IHA法检测血清结果,包括时间、地区、宿主动物分布情况。结果1996-2016年共采用IHA法检测各种宿主动物血清38096份,检出阳性172份,阳性率为0.45%;21个年度中除2001、2015年外,其余19个年度均通过IHA法判定有动物鼠疫的发生和流行;25个旗(县、市)中有10个通过IHA法检出阳性血清;共6种宿主动物血清中IHA法检出阳性结果,其中啮齿类动物5种、食肉类1种,大沙鼠阳性率达6.17%(91/1475),检出宿主动物血清阳性最高滴度达1:10240。结论 IHA法易于操作、经济合算,在内蒙古长爪沙鼠疫源地系统监测中为及时发现疫情提供了巨大帮助。

Objective To analyze and evaluate the application of indirect hemagglutination test (IHA) method in the epidemic area of Meriones unguiculatus in Inner Mongolia.Methods The plague monitoring summary and epidemic analysis reports of each year in Inner Mongolia from 1996 to 2016 were collected.The serum results of the IHA method were analyzed,including the time,region and the distribution of host animals.Results From 1996 to 2016,a total of 38096 serum samples were detected from various host animals,and 172 positive samples were detected,with a positive rate of 0.45%.In the 21 years,except 2001 and 2015,the occurrence and prevalence of plague were judged by the IHA method for the remaining 19years.There were 10 counties detected positive sera by IHA method;the positive results were detected by IHA method in 6 kinds of host animals,including 5 rodents and 1carnivorous,and the positive rate of Rhombomys opimus was up to 6.17%(91/1475),the highest titer of the seropositive host animals was as high as 1:10240.Conclusions The IHA method is easy to operate and economical.It plays a huge role in the timely detection of the epidemic in systematic monitoring of the Meriones unguiculatus in Inner Mongolia.