淫羊藿根薄层色谱鉴别方法的建立

Development of a TLC Method for the Identification of Epimedium Root

目的:建立淫羊藿根的薄层色谱鉴别方法。方法:样品通过超声30 min,蒸干后加甲醇溶解作为供试品溶液,另取淫羊藿苷和朝藿定C加甲醇制成1 mg/m L的溶液,作为对照品溶液。将供品溶液、对照品溶液各5μL点于硅胶G板,以氯仿:甲醇:水(13. 5∶6∶2)下层溶液为展开剂,展开后,喷以10%硫酸乙醇溶液,105℃加热至斑点清晰。结果:所得的薄层色谱图谱检出淫羊藿苷和朝藿定C,且斑点清晰,专属性强。结论:该方法可作为淫羊藿根的薄层鉴别方法。

Objective To develop a TLC method for the identification of Epimedium root.Method The samples were extracted by ultrasound for 30min,then dried by distillation,finally dissolved in methanol as the sample solution .And icariin and epimedin C were dissolved in methanol solution at 1mg/ml as the reference solution.5μL of the sample solution and reference solution were load- ed onto silica gel G plates respectively,and the lower solution of chloroform:methanol:water (13.5:6:2)was used as developing solvents.After development,10%sulfuric acid ethanol solution was sprayed,and the plate was heated at 105℃ till spots were clearly shown.Result Spots of icariin and epimedin C were clearly detected in the thin-layer chromatography and showed good specialization. Conclusion The present method can be used in the thin-layer identification of Epimedium roots.