MSTN基因rs7570532多态性对全身振动训练干预绝经后女性体成分效果的影响及分子机制

Influence of Myostatin Gene rs7570532 Polymorphism on Body Composition in Postmenopausal Women After Whole Body Vibration Training and the Molecular Regulation Mechanism

目的:对绝经后女性进行为期3个月的全身振动训练,观察其对绝经后女性体成分的影响,分析肌肉生长抑素(myostain,MSTN)基因rs7570532位点多态性对振动训练干预体成分效果的影响,并探讨其分子调控机制。方法:从社区招募60名汉族健康绝经后女性,年龄为(58. 87±4. 70)岁,对其进行为期3个月的全身振动训练,分别在振动训练干预前、后测试受试者的身高、体重、体重指数(body mass index,BMI)以及体成分,体成分的测试采用Prodigy Lunar双能X线骨密度仪,测试指标包括全身脂肪百分比(percentage of fat mass,FM%)、全身脂肪含量(fat mass,FM)和全身瘦体重含量(lean mass,LM)。采用飞行质谱法对MSTN基因rs7570532多态位点进行基因分型;采用荧光素酶报告系统检测不同基因型重组质粒报告基因的相对活性。结果:绝经后女性经过3个月的全身振动训练,其体重、BMI、FM%和FM均显著下降,LM无显著变化。受试者基因分型结果显示,34人为TT型,19人为TC型,仅有2人为CC型,受试者基因型频率符合H-W遗传平衡定律。经过3个月的全身振动训练,TT型受试者的FM%显著下降(36. 4±5. 4,35. 2±5. 0,P <0. 01),FM显著下降(22. 6±5. 5,21. 7±5. 3,P <0. 01),LM显著上升(36. 8±3. 6,37. 2±3. 9,P <0. 05); TC+CC型受试者的体重和体成分各指标均无显著变化。在控制干预前受试者各项指标的基础值后,TT型和TC+CC型受试者之间FM%和LM具有显著差异,FM的差异性消失。p GL3-promoter-MSTN-T重组质粒报告基因的相对活性(3. 30±1. 12)显著高于p GL3-promoter-MSTN-C的(1. 76±0. 92,P <0. 01),且2者均显著高于空质粒对照组(0. 81±0. 17,P <0. 01)。结论:1) 3个月的全身振动训练可显著改善绝经后女性的体重和体成分; 2) MSTN基因rs7570532位点多态性显著影响振动训练干预体成分的效果; 3)此位点多态性可显著影响下游基因的表达活性,其可能是振动训练改善体成分效果个体差异的原因之一。

Objective: To observe the effect of 3-month whole body vibration ( WBV) training on body composition in postmenopausal women,to analyze the intervention effect of myostain ( MSTN) gene rs7570532 polymorphism on it,and to explore the molecular regulation mechanism. Methods: Sixty healthy postmenopausal women ( 58. 87 + 4. 70 yrs) of Han nationality were recruited from communities. They were trained by WBV for three months. Their height,weight,body mass index ( BMI) and body composition were measured before and after the intervention. The body composition was measured by Prodigy Lunar dual energy X-ray bone,including the indexes of body fat percentage ( FM%) ,body fat mass ( FM) ,and body lean mass ( LM) . The rs7570532 polymorphic site of MSTN gene was genotyped by Matrix-assisted laser desorption /ionisation time-offlight mass spectrometry ( MALDITOFMS) technology,and the relative activity of recombinant plasmid reporter genes of different genotypes was detected by luciferase reporting system. Results: After 3-month WBV training,women’s body weight,BMI,FM% and FM decreased while LM had no change. All the people were divided into three genotype groups: TT ( n = 34) ,TC ( n = 19) and CC ( n = 2) ; this genotype frequency of subjects accorded with H-W's law of genetic equilibrium. After 3-month WBV training,the FM% ( 36. 4 ± 5. 4,35. 2 ± 5. 0,P < 0. 01) and FM ( 22. 6 ± 5. 5, 21. 7 ± 5. 3,P < 0. 01) of subjects with TT allele significantly decreased,and their LM ( 36. 8 ± 3. 6,37. 2 ± 3. 9,P < 0. 05) increased,; there were no significant changes in all parameters in subjects with TC and CC alleles. After controlling the baselines of the subjects,there were significant difference in FM% and FM between the TT and the TC + CC group,and the difference in FM disappeared. The relative luciferase activity of the reporter gene in recombinant vector carrying the T allele was 3. 30 ± 1. 12,markedly higher than that in recombinant vector carrying the C allele ( 1. 76 ± 0. 92,P < 0. 01) ,and both of them were higher than the control group ( 0. 81 ± 0. 17,P < 0. 01) . Conclusions: 1) The 3-month WBV could significant improve the body weight and body composition in postmenopausal women. 2) The subjects with TT genotype of the rs7570532 polymorphism could affect the effect of WBV training on body composition. 3) This polymorphism could significantly affect the expression activity of downstream genes,which may be one of the reasons for individual differences in the effect of vibration training on improving body composition.