携IR780碘化物液态氟碳纳米粒造影剂的制备及体外双模态成像

Preparation and in vitro dual-mode imaging of IR780-loaded liquid fluorocarbon nanoparticles

目的制备携带IR780碘化物的液态氟碳纳米粒(IFNPs),观察其体外超声/光声双模态成像效果。方法以羟基端乳酸/羟基乙酸共聚物(PLGA-COOH)、IR780碘化物和全氟戊烷(PFP)为原料,采用双乳化法制备PLGA包裹的纳米粒IFNPs。以光学显微镜、共聚焦显微镜、透射电子显微镜及扫描电子显微镜检测其一般物理特性;以马尔文粒径分析仪分析其粒径大小、分布及表面电位;以紫外分光光度计测定IFNPs中IR780的包封率;观察IFNPs体外光声成像和超声成像的能力。结果成功制备出IFNPs,其形态规则,大小均一,透射电子显微镜下表现为外壳黑色、内部灰白色的球形结构,扫描电子显微镜显示纳米粒表面光滑。IFNPs粒径为(241.87±3.47)nm,电位为(-0.766±0.096)mV,IR780包封率为(90.38±0.48)%。随IFNPs浓度增加,体外光声成像及超声成像效果均显著增强。结论携带IR780碘化物的液态氟碳纳米粒造影剂制备成功,可用于体外超声/光声双模态成像。

Objective To prepare IR780-loaded liquid fluorocarbon nanoparticles (IFNPs),and to observe their ability in photoacoustic and in vitro ultrasound imaging.Methods IFNPs were prepared using double emulsion method with PLGA- COOH,IR780and perfluoropentane (PFP).IFNPs'general physical properties were detected with optical microscope, confocal microscopy,transmission electron microscope (TEM)and scanning electron microscope (SEM).The size, distribution and zeta of IFNPs were measured with Malvern particle size analyzer.The entrapment efficiency of IR780in IFNPs was observed with ultraviolet spectrophotometer.The ability of IFNPs in photoacoustic and in vitro ultrasound imaging using was evaluated.Results IFNPs presented with homogenized size distribution.TEM images showed a spherical structure with black shell and grey core.SEM images demonstrated the smooth surface and regular shape.The mean size of IFNPs was (241.87±3.47)nm,mean zeta was (-0.766±0.096)mV.The entrapment efficiency of IR780 in IFNPs was (90.38±0.48)%.The enhancement of IFNPs improved with the increased concentration in photoacoustic and in vitro ultrasound imaging.Conclusion IFNPs can be successfully prepared and used for photoacoustic and in vitro ultrasound imaging.