黑木耳培养新基质配方优化及β-寡聚酸应用

Black Fungus Develop New Optimization Substrate and β-Oligomeric Acid Application

以冀诱5号、杂3木耳为出发菌株,对比以柿木屑为主料、香蒲为辅料的新基质培养基中,黑木耳菌丝营养生长;并研究了生物制剂β-寡聚酸的应用效果。结果表明:每100 g新基质培养基中香蒲占10%时,菌丝生长速度最快,与对照组有显著性差异;在PDA中加入不同量的β-寡聚酸,研究不同量的β-寡聚酸对冀诱5号、杂3木耳菌丝生长的影响和发酵醪液中多糖含量的影响。结果表明:每500 mL PDA培养基加入0.5 mLβ-寡聚酸时,菌丝生长速度最快,与对照组有显著性差异。在加入β-寡聚酸效果最好处理浓度即每500 mL PDA中加入0.5 mL β-寡聚酸下的发酵醪液多糖含量有显著提高,与对照组有显著性差异。

Taking the cockroach 5 and miscellaneous 3 fungus as the starting strains, the vegetative growth of black fungus hyphae was studied in the new substrate medium with the main ingredients of persimmon wood chips and cattail as the starting strain, and the application effect of the biological preparation β-oligomeric acid was studied. The results showed that the mycelial growth rate was the fastest when the cattails accounted for 10% of the new substrate medium, which was significantly different from the control group. Different amounts of β-oligomeric acid were added to the PDA to study different amounts of β-oligomreic acid. The effect of β-oligomreic acid on the growth of mycelium of Jiyou 5, Za 3 fungus and the content of polysaccharide in fermentation mash. The results showed that the mycelium growth rate was the fastest when 0.5 mL of β-oligomeric acid was added per 500 mL of PDA medium, which was significantly different from the control group. In the best treatment concentration of β-oligomeric acid, the content of polysaccharide in the fermentation sputum was significantly increased in 0.5 mL of β-oligomeric acid per 500 mL PDA, which was significantly different from the control group.