响应面法优化甜叶菊萃取液絮凝工艺

Optimization of Flocculation Process of Stevia Extract by Response Surface Methodology

为优化甜叶菊萃取液的絮凝工艺,考察不同絮、助凝剂搭配及用量对絮凝效果的影响,考察絮凝条件对絮凝效果的影响。在单因素试验基础上,选取反应温度、反应时间、反应液pH为影响因子,以脱色率为响应值,应用中心组合Box-Behnken试验和响应面分析法,模拟得到二次多项式回归方程的预测模型,进行响应面分析。结果表明,经优化后的甜叶菊萃取液最佳絮凝工艺条件为:絮凝反应温度52℃,反应液pH 9.5,絮凝反应时间36 min,在此条件下得到甜叶菊萃取液经絮凝后脱色率平均值为97.72%,甜菊糖苷损失率为2.65%。

In order to optimize the flocculation process of Stevia extract, the effects of different flocculation, coagulant and dosage on flocculation were investigated. The effect of flocculation on flocculation was investigated. On the basis of single factor experiment, reaction temperature, reaction time, reaction liquid pH for impact factors, the decolorization rate as the response value, application center combination Box-Behnken experiment and response surface analysis, the prediction model of quadratic polynomial regression equation was simulated and was analyzed through the response surface method. The results showed that the best flocculation process conditions for Stevia extract was 52 ℃ flocculation reaction temperature, reaction liquid pH 9.5, the flocculation reaction time of 36 min. On such condition, stevia extract after flocculation decolorization rate was 97.72%, and Stevia glycoside loss rate was 2.65%.