鲤在低温胁迫下肝胰腺转录组测序分析

Transcriptome Sequencing and Analysis of Hepatopancreas from Carps Under Cold Stress

检测低温耐受与低温敏感鲤品种低温胁迫下的差异表达基因,探究鲤低温适应的分子应答过程。低温胁迫后对松浦镜鲤及荷包红鲤肝胰腺进行转录组测序,并对表达差异基因进行功能、通路富集分析。通过差异表达分析,共获得10 521个差异表达基因,其中5 246个基因仅在低温耐受品种发生表达变化。功能、通路富集分析将差异基因显著富集在包括糖酵解/糖质新生代谢途径在内的144个代谢途径。选取5个基因进行qRT-PCR验证,定量结果与RNA-seq结果相关性达0.89。研究结果表明低温胁迫会显著改变鲤肝胰腺的转录活动,编码糖酵解/糖质新生途径的多个关键酶基因在低温耐受品种的表达量显著高于低温敏感品种,推测这些基因功能有助于其长期低温适应。

This work aims to detect the differentially-expressed genes(DEGs)of cold-tolerant and cold-sensitive carps and tounderstand the molecular response mechanisms for carps to adapt in cold environment. The transcriptome sequencing of hepatopancreas fromcold-tolerant Songpu carp and a cold-sensitive Hebao carp were conducted and the enrichment analysis of DEG function and pathways werecarried out. Totally,10 521 DEGs were detected via differentially-expressed analysis,and 5246 DEGs of them presented expression in coldtolerant species. DEGS were enriched in 144 metabolic pathways including glycolysis/gluconeogenesis pathway. Five DEGs were selected toconduct qRT-PCR,and the correlation of the results between qRT-PCR and RNA-seq was 0.89.This study revealed that cold stress significantlychanged the transcriptome of hepatopancreas in the experimental carp. The expressions of genes encoding the key enzymes of glycolysis/gluconeogenesis pathway were apparently higher than that in cold-sensitive ones,revealing that the functions of these genes facilitate the longterm cold-adaptation of the cold-tolerant carps.