闰年霉素分离纯化工艺研究

Studies on the separation and purification of lipiarmycin

目的开发闰年霉素的分离纯化工艺。方法采用二元液相分离系统,以反相填料为载体,对填料的类型和洗脱条件进行优化,得到适宜的操作条件。结果反相硅胶C18为最佳层析介质,洗脱剂为50%乙腈-酸水,上样量为10mg/m L填料。在此实验条件下所得产品纯度≥98.5%,纯化收率≥70%。结论此种闰年霉素的纯化方法简单易行,收率稳定,适于产业化应用。

Objective To develop the separation and purification processes of lipiarmycin.Methods Using a binary liquid chromatography system,the separation and purification processes of lipiarmycin were studied by optimizing the different types of chromatography fillers and elution conditions to obtain a suitable operating conditions.Results The results showed that the type of reverse phase silica gel C18 was selected as the best chromatography filler,50% acetonitrile-H2O was used as the eluent,and the amount of sample was 10mg/mL filter. The purity of the final product was more than 98.5% and the yield of lipiarmycin was above 70% under the optimal conditions.Conclusion The method is simple and available for the commercial process.