谷朊蛋白源抗氧化肽的活性研究

A study on the antioxidant activities of peptide from gluten protein

以总抗氧化能力(FRAP)、DPPH和OH自由基清除能力为评价指标,采用木瓜蛋白酶和碱性蛋白酶分步水解谷朊蛋白,并采用超滤、DA201-C大孔树脂和凝胶Sephadex G-15富集谷朊蛋白水解液中的高活性抗氧化肽。实验结果显示,谷朊蛋白经酶解及进一步的初步分离获得了组分GF2,其FRAP值、DPPH·清除和·OH清除IC_(50)值分别达到0. 893 mmol/L、6. 13 mg/mL和0. 472 mg/mL。结果表明:超滤、DA201-C大孔树脂和凝胶Sephadex G-15分离有效富集了酶解液中的高抗氧化活性肽。

In order to obtain the highly active antioxidant peptides,gluten protein was hydrolyzed by papaya protease and alkaline protease.FRAP (total antioxidant capacity),DPPH ·and ·OH scavenging activities were used as evaluation index.Ultrafiltration,macroporous resin DA201-C and gel Sephadex G-15 were used to enrich the peptides with high antioxidant activity in the hydrolysates obtained from gluten protein.The results showed that the component GF2 with strong antioxidant capacity was obtained,and the value of FRAP,the IC50 of DPPH ·and ·OH scavenging activity were 0.893mmol/L,6.13mg/mL,and 0.472mg/mL,respectively.