龙牙百合贮藏期间内源激素的变化

The changes of endogenous hormones of 'Longya' Lily during storage period

以龙牙百合的试管鳞茎及商品鳞茎为材料,研究了5℃低温与25℃常温贮藏对鳞茎内外层鳞叶的内源激素(ABA、GA_3和IAA)含量的动态变化影响,实验结果如下:在5℃低温贮藏过程中,GA_3含量最高,IAA次之,ABA较低,且GA_3与IAA含量呈明显上升趋势,而ABA含量总体呈下降趋势;在25℃常温贮藏过程中,ABA、GA_3和IAA的含量与5℃低温贮藏的变化基本一致,只是变化的幅度较小。同一鳞茎内层鳞叶和外层鳞叶的同种激素含量基本处在同一水平,变化幅度基本一致。试管鳞茎ABA的含量要比商品鳞茎的要高,而GA_3和IAA的含量则比商品鳞茎的要低。贮藏过程中内源激素GA_3/ABA、IAA/ABA的比值总体呈上升趋势,贮藏后期GA_3/ABA比值急剧上升。同时,探讨了内源激素的变化和平衡与休眠解除的关系,认为休眠的解除可能为GA_3类促进物质和ABA类抑制物质相互作用的促进抑制平衡所致。

The content of endogenous hormones(ABA,GA3 and IAA)in inner and outer scales were studied by HPLC when the Liliumbrownievar.viridulum Baker bulbs in vitro and the common bulbs were stored in 5℃and 25℃.The results are followings.When the bulbs were stored in 5 ℃,the contents of GA3 and IAA were higher than that of ABA,and the GA3 contents was the highest.The contents of GA3 and IAA increased in evidence,while ABA decreased all the times especially at the end of the storing.When the bulbs were stored in 25℃,the changes of these three hormones were similar to those in 5℃,while the scopes of the changes were smaller than those in 5℃.The contents of the same kinds of hormone were at the similar level between inner and outer scales in one bulb,and the change scopes were similar also.The ABA content of the bulbs in vitro was higher than that of common bulbs,while the contents of GA3 and IAA were lower.So there were some difference in the speed of relieving dormancy,and the common bulbs were more quickly.The changes of GA3/ABA and IAA/ABA increased mostly to high levels,and the GA3/ABA increased rapidly at the end of storing.At the same time,the relation was studied between endogenous hormones and relieving dormancy.The results indicated that the reciprocity between some stimulative molecules like GA3 and some inhibiting molecules like ABA was probably the most factor in relieving dormancy of the bulbs.