链霉蛋白酶对幽门螺杆菌感染患者胃癌组织MUC1及MUC5AC的影响

Effect of pronase on MUC1 and MUC5AC of gastric cancer patients with Helicobacter pylori infection

目的探讨链霉蛋白酶对幽门螺杆菌(Helicobacter pylori,H.pylori)感染患者的胃癌组织MUC1及MUC5AC的影响。方法取诊断为胃癌且呼气试验阳性患者的术后标本,置于生理盐水、链霉蛋白酶及乙酰半胱氨酸中浸泡30 min,经免疫组化染色后制成切片,观察MUC1及MUC5AC的表达,并判定结果。结果经过链霉蛋白酶及乙酰半胱氨酸处理过的MUC1及MUC5AC水平降低,链霉蛋白酶组MUC1较生理盐水组MUC1比例增加,且两组MUC1/MUC5AC比例增加。结论链霉蛋白酶可以降低H.pylori感染的胃癌患者MUC1和MUC5AC的水平,链霉蛋白酶可以破坏H.pylori赖以生存的胃黏液凝胶层水平。经过链霉蛋白酶处理后,黏液层抗H.pylori的能力可能会增强。这为根除H.pylori提供了一种新的思路。

Objective To investigate the effect of pronase on MUC1 and MUC5AC of gastric cancer patients with Helicobacter pylori(H.pylori) infection.Methods The specimens of the patients with positive gastric cancer and positive breath test were soaked in saline,pronase and acetylcysteine for 30 minutes,and the expressions of MUC1 and MUC5AC were observed and the results were determined.Results The levels of MUC1 and MUC5AC treated by pronase and acetylcysteine were decreased,and the proportion of MUC1 in the pronase group was increased compared with the MUC1 in the saline group,and the proportion of MUC1/MUC5AC was increased in the two groups.Conclusion Pronase can reduce the levels of MUC1 and MUC5AC in patients with H.pylori infection,and pronase can destroy the level of gastric mucous gel layer on which H.pylori is dependent.After being treated with streptomyces,the ability of mucus layer to fight H.pylori is likely to increase.All these provide a new way to eradicate H.pylori.