慢性牙周炎患者牙龈组织中IL-6、IL-34和M-CSFR的表达及临床意义

Expression of cytokines Il-6, IL-34 and M-CSFR in chronic periodontitis and its clinical significance

目的:比较慢性牙周炎患者牙龈组织和健康牙龈组织中IL-6、IL-34及M-CSFR的表达水平,探讨IL-34在慢性牙周炎发病中的作用。方法:收集8例诊断为轻度慢性牙周炎患牙的牙龈组织和8例诊断为重度慢性牙周炎患牙的牙龈组织作为病例组,8例冠延长手术切除的牙龈组织作为对照组,应用实时荧光定量PCR检测IL-6、IL-34及M-CSFR mRNA的表达;应用蛋白免疫印迹检测IL-6、IL-34及M-CSFR蛋白的表达。使用GraphPad Prism 6.0对结果进行统计学分析。结果:IL-6、IL-34、M-CSFR mRNA及蛋白在慢性牙周炎牙龈组织中的表达水平均显著高于正常牙龈组织中的表达(P<0.05)。结论:IL-6、IL-34及M-CSFR的表达可能与慢性牙周炎密切相关。

PURPOSE:To detect the expression of IL-6,IL-34 and M-CSFR in chronic periodontitis and healthy gingival tissue and discuss the role of IL-6,IL-34 and M-CSFR in the etiology of chronic periodontitis.METHODS:A total of 8 patients with mild chronic periodontitis and 8 patients with severe chronic periodontitis were collected in this study.As control,8 normal gingival tissues from extracted healthy were selected.The expression of IL-6,IL-34 and M-CSFR mRNA was detected by real-time PCR; the expression of IL-6,IL-34 and M-CSFR protein was detected by Western blotting.Statistical analysis was performed using GraphPad Prism 6.0.RESULTS:The levels of IL-6,IL-34,M-CSFR mRNA and protein expression in chronic periodontitis was significantly higher than that of the normal gingival tissue(P<0.05).CONCLUSIONS:IL-6,IL-34 and M-CSFR may be closely related to the development of chronic periodontitis.