纳米ZnO对微藻的毒性效应和生物富集的研究

Toxicity and Bioaccumulation of Zinc Oxide Nanoparticles by Microalgaes

文章研究了纳米ZnO对小球藻和羊角月牙藻的毒性效应和生物富集与转化效率。纳米ZnO对小球藻和羊角月牙藻细胞的96 h-EC_(50)值分别为17.11和2.26 mg/L。彗星电泳图中拖尾的DNA反映出纳米ZnO诱导了小球藻和羊角月牙藻细胞的DNA损伤,产生了基因毒性。小球藻和羊角月牙藻对培养基中总锌的去除率随时间的延长而增加,在处理96 h后其去除效率可分别达到60.8%和24.1%。双因素方差分析结果表明(P<0.001),小球藻对纳米ZnO具有更强的富集效率。

The toxicity, removal and bioaccumulation of zinc oxide nanoparticles(nano-ZnO) by two unicellular green microalgal species, Chlorella vulgaris and Selenastrum capricornutum, were studied. The cytotoxicity of nano-ZnO was concentration-, species-and time-dependent. The 96 h-EC50 values of nano-ZnO to C. vulgaris and S. capricornutum were17.11 and 2.26 mg/L, respectively, suggesting that C. vulgaris was much more tolerant to nano-ZnO than S. capricornutum.The unclear tail in comet assay images reflected that nano-ZnO induced DNA damage in both microalgae. Two-way ANOVA results showed that C. vulgaris had higher removal efficiencies and uptake ability of nano-ZnO than S. capricornutum(P<0.001). Further research is needed to understand the toxicity mechanism of nano-ZnO and the trophic transfer effect of nano-ZnO through the food chain.