磷脂酶固定化载体材料的研究进展

Progress in carrier materials for immobilization of phospholipase

游离磷脂酶价格昂贵且稳定性差限制了其应用,固定化技术使酶稳定性增强,可以反复使用,降低了成本,极大地拓宽了酶的应用范围。性能优良的固定化酶应具备成本低、稳定性高和催化活性高等特性。载体材料不仅是固定化酶的重要组成部分,还是影响固定化酶性能的因素之一。对磷脂酶固定化载体材料主要从无机材料、有机材料、复合材料以及无载体4个方面进行综述,并对磷脂酶固定化载体材料发展方向予以展望,为固定化磷脂酶研究在载体材料上的选择提供了参考依据。

Expensive price and instability of the soluble phospholipases limit their application.Immobi-lization technology enhances the stability of the enzyme.The repeated use of the immobilized enzyme decreases the cost and greatly broadens the scope of application of the enzyme.The immobilized enzyme with excellent performance should have the characteristics of low cost,high stability and high catalytic activity.The carrier material is not only an important part of the immobilized enzyme,but also one of the factors affecting the performance of the immobilized enzyme.The carrier materials of phospholipases immobilization,such as inorganic materials,organic materials,composite materials and no carrier were re-viewed.The future prospects of carrier materials were also addressed with the aim to provide a certain ref-erence in the choice of materials in immobilization of phospholipase.