新疆乌苏市古尔图地区长尾黄鼠感染巴尔通体情况调查

Analysis on Bartonella infection status of Citellus undulatus in Wusu city Guertu township, Xinjiang

目的了解新疆维吾尔自治区(新疆)乌苏市古尔图地区长尾黄鼠的巴尔通体感染状况,为该地区鼠传疾病风险预警与防控措施制定提供科学依据。方法 2017年5-9月采用一日弓形夹法在查岗果勒、布兰布拉克、白石头等地捕获长尾黄鼠86只,无菌采集鼠体肾脏,试剂盒法提取全基因组DNA。应用实时荧光定量PCR法检测样本DNA巴尔通体特异性基因ssrA,计算感染率,分析样本的检测循环阈值(cp值)。结果 42只长尾黄鼠的巴尔通体实时荧光定量PCR检测为阳性,阳性率为48.84%;阳性样本cp值最低为27.49,最高为37.83,其中88.10%阳性样本的cp值在35.00～37.83之间,查岗果勒、布兰布拉克、白石头三地巴尔通体的阳性率分别为48.48%、60.87%和40.00%;布兰布拉克地区巴尔通体感染率、阳性动物染蚤率、蚤指数均高于查岗果勒和白石头地区;三地巴尔通体感染率与动物染蚤率差异无统计学意义(P>0.05)。结论新疆乌苏市古尔图地区长尾黄鼠间存在巴尔通体自然感染,传播范围广,但群体带菌量较低。

Objective To understand the infection status of Bartonella in Citellus undulatus from Guertu,and to provide the basis for risk early warning and control measures of rodent-borne diseases.Methods In total,86 C.undulatus were collected from Chagangguole,Bulanbulake and Baishitou by bow-shaped clip method during 24 hours,the kidneys of the captured rodents were sampled under aseptic condition,genome DNA was extracted and detected by real-time fluorescence quantitative PCR targeting ssrA of Bartonella.The rate of infection were calculated and analyzed by cp test (cp value). Results A total of 86 C.undulatus were tested,the infection rate of Bartonella in those rodents was 48.84%(42/86).The cp of positive samples had the highest value of 37.83 and the lowest value of 27.49,88.10% of positive samples was in the "35.00-37.83"range.The infection rate of Chagangguole,Bulanlulake and Baishitou were 48.48%,60.87%,40.00%, respectively.The flea infection rate and flea index in Bulanlulake were higher than those of other places,the infection rate had no correlation with the flea infection in Guertu (P>0.05).Conclusion There were natural infection of Bartonella among C.undulatus in Guertu,with tendency of wide spread,but pathogen loads were relatively low in wild rodents.