微浓度过氧化氢的快速分光光度法检测

A rapid determination method for microconcentration of hydrogen peroxide by spectrophotometry

建立了一种采用分光光度计快速测定溶液中微浓度过氧化氢的方法。该方法采用10-乙酰基-3,7-二羟基吩嗪在过氧化氢酶作用下与过氧化氢快速反应生成9-羟基-3-异吩嗪酮,然后于570 nm下完成吸光度测定,根据吸光度值与过氧化氢浓度间的线性关系确定溶液中过氧化氢的浓度。结果表明,显色反应的最佳条件为:n(10-乙酰基-3,7-二羟基吩嗪)/n(H2O_2)≥5. 0,暗反应时长≥8. 0 min;方法重现性测试的相对标准偏差为0. 16%,准确性测试的相对标准偏差≤0. 78%。该方法具有操作简单、反应灵敏且迅速、稳定性和重现性好及检测准确度高等优点,不仅适用于工业生产快速分析,也能满足检测低浓度过氧化氢的要求。

A spectrophotometric method is established to determine rapidly micromolar of hydrogen peroxide in a solution.This method bases on the rapid reaction between 10-acetyl-3,7-dihydroxyphenazine and hydrogen peroxide under catalase,which produces 7-hydroxy-3-phenazin-3-one that absorb strongly light with a wavelength of 570 nm.According to the linear quantitative relationship between the absorbance and concentration of hydrogen peroxide,the concentration of hydrogen peroxide in the solution can be calculated. It is found that the best conditions for chromogenic reaction are as follows: n(10-acetyl-3,7-dihydroxyphenazine)/n(H2 O2) is more than 5. 0 and reaction time is longer than eight minutes in the dark.This method is of excellent stability,accuracy and accuracy performance.The relative standard deviation of the reproducibility and accuracy tests is 0. 16% and no more than 0. 78%,respectively. This method exhibits simple,sensitive,rapid and accurate. It can either be used for the rapid industrial analysis or satisfy the determination requirements of low concentration hydrogen peroxide.