几种支原体检测试剂的临床应用评价

Clinical application and evaluation of multiple mycoplasma detection reagents

目的评价检测试剂支原体鉴定试剂盒对支原体的临床诊断价值。方法选用支原体鉴定试剂盒支原体培养鉴定计数药敏试剂盒、解脲支原体(UU)核酸测定试剂盒(荧光PCR法)检测369份白带标本和宫颈标本。结果支原体鉴定试剂盒与支原体培养鉴定计数药敏试剂盒及解脲支原体qPCR对解脲脲原体检测的灵敏度和符合率分别为91.2%、87.0%和83.8%、86.7%。Kappa检验一致性分析结果分别为0.73、0.71,表明新型试剂的检测结果与培养法和PCR检测的结果具有高度的一致性。支原体鉴定试剂盒与支原体计数药敏培养液对人型支原体检测的灵敏度和符合率分别为87.3%、97.3%。Kappa检验一致性分析结果为0.889,表明新型试剂的检测结果与培养法检测的结果具有最强的一致性。结论支原体鉴定试剂盒操作简便、快速,与培养法和PCR检测符合度较高,可以满足临床对该项目灵敏度、特异性的要求。

Objective To evaluate the clinical diagnostic value of mycoplasma identification kit for mycoplasma.Methods A total of 369 leucorrhea specimens and cervical specimens were detected by using the mycoplasma identification kit,the mycoplasma culture identification and sensing kit provided by Antu Bioengineering Co.,Ltd.,ureaplasma urea nucleic acid assay kit (fluorescence PCR method)provided by Shanghai Zhijiang.Results The sensitivity and coincidence rate of mycoplasma identification kit,mycoplasma culture identification and drug sensitivity kit and PCR for Ureaplasma urealyticum Uu detection were 91.2%,87.0% and 83.8%,86.7%,respectively.The Kappa test consistency was 0.73 and 0.71,respectively,indicating that the detection results of the new reagents were highly consistent with the results of the culture method and the PCR test.The sensitivity and coincidence rate of mycoplasma identification kit and mycoplasma susceptibility culture solution for detection of human mycoplasma were 87.3% and 97.3%,respectively.The Kappa test consistency was 0.889, indicating that the detection results of the new reagents showed the strongest consistency with the results of culture assays. Conclusion The mycoplasma identification kit is simple and rapid to operate,and has a high degree of conformity with culture methods and PCR detection,which can meet the requirements of clinical sensitivity to theproject.