摘要
目的对广州市禽类市场外环境H7N9禽流感病毒流行情况和基因进化特点进行监测分析,为人感染H7N9禽流感防控提供研究数据。方法采集2014—2017年广州市禽类市场外环境标本,采用实时荧光定量RT-PCR方法检测H7N9禽流感病毒并绘制血凝素(HA)基因遗传进化树,分析H7N9禽流感病毒流行特点和基因进化特点。结果 2014—2017年累计检测外环境标本28 252份,检出H7N9阳性标本888份,阳性率为3.14%。检出率2015年最低,2014年最高,分别为1.60%和3.89%。2—5月出现H7N9禽流感病毒流行高峰,4月检出率最高为13.00%。测序分析结果显示,HA基因核苷酸同源性为88.6%~100%,氨基酸同源性为92.0%~100%。裂解位点分析发现,2017年外环境开始出现高致病性H7N9病毒。进化分析发现,HA基因呈现多进化分支特点。结论 2014—2017年广州市禽类市场外环境H7N9禽流感病毒持续存在且病毒变异较快,呈现基因多样性。2017年外环境已发现高致病性H7N9病毒,近年病毒HA基因归属于华南分支,呈现本土化,同时监测到来源于野鸟的另一独立分支的病毒存在,提示加强本地区野鸟H7N9禽流感病毒监测的必要性。
Objective To understand the circulation and gene evolution of avian influenza A (H7N9)virus in Guangzhou and provide research data for prevention and control of human infection with avian influenza A (H7N9)virus.Methods The samples were collected in poultry markets in Guangzhou during 2014-2017.Avian influenza A (H7N9)virus was detected by real-time RT-PCR,and the HA gene was plotted for phylogenetic tree.Results A total of 28252 samples were detected,in which 888 were positive for H7N9 virus (3.14%).The circulation peak of H7N9 virus occurred during February-May.The highest detection rate was 13% in April.The nucleotide homology of HA gene was 88.6%-100%,and the amino acid homology was 92.0%-100%.The highly pathogenic avian influenza A (H7N9)virus has been detected in the environment in 2017.Evolutionary analysis revealed that the HA gene exhibited multiple evolutionary branching characteristics.In recent years,the virus HA gene has been localized,belonging to the Southern China branch.And we have detected the isolate belonging to other evolution branch from wild birds.Conclusion Avian influenza A (H7N9)virus continued to exist in Guangzhou during 2014-2017,and the virus mutated rapidly.It is necessary to strengthen the surveillance for avian influenza A (H7N9)virus in wild birds.
作者
曹蓝
李魁彪
刘艳慧
陈艺韵
陆剑云
鲁恩洁
刘文辉
夏丹
李铁钢
王鸣
狄飚
杨智聪
Cao Lan;Li Kuibiao;Liu Yanhui;Chen Yiyun;Lu Jianyun;Lu Enjie;Liu Wenhui;Xia Dan;Li Tiegang;Wang Ming;Di Biao;Yang Zhicong(Guangzhou Municipal Center for Disease Control and Prevention,Guangzhou 510440,Guangdong,China)
出处
《疾病监测》
CAS
2018年第11期897-901,共5页
Disease Surveillance
基金
广州市医药卫生科技项目(No.20181A011052)
广东省医学科研基金(No.A2018196)
广州市卫生和计划生育科技项目(No.20181A011051)
广州市医学重点学科建设项目(授权号:2017–2019–07)
国家科技重大专项(No.2017ZX10103011–005)~~
关键词
禽流感
H7N9
监测
遗传进化
Avian influenza virus
H7N9
Survey
Genetic evolution