云克针剂诱导兔骨关节炎模型软骨修复的实验研究

Experimental Study on the Repair of Cartilage in Rabbit Osteoarthritis Model Induced by 99TC-MDP Injection

目的:建立兔膝骨关节炎模型,通过在兔膝关节腔内注射云克(99TC-MDP)针剂,观察兔膝关节内软骨病理学结构的改变。方法:32只体质量(2.0±0.2)kg健康新西兰成年大耳雄性白兔随机分为正常组、空白对照组、静脉注射组、关节腔注射组。正常组不予处理,空白对照组、静脉注射组、关节腔注射组通过对兔膝关节内侧半月板前1/3切除及前交叉韧带(ACLT)切断制备骨关节炎(OA)模型法造模。静脉注射组模型每日静脉注射云克针剂0.2mL,连续5周;关节腔注射组模型每周膝关节腔内注射云克针剂0.2mL,1周1次,给药5周,正常组、模型组不予处理。第6周观察各组软骨细胞病理学变化。结果:苏木精-伊红染色观察,正常组关节软骨Mankin评分高于空白对照组、静脉注射组、关节腔注射组;静脉注射组、关节腔注射组关节软骨病理评分高于空白对照组;静脉注射组、关节腔注射组关节软骨Mankin评分差异无统计学意义(P<0.05)。电镜超微结构观察静脉注射组、关节腔注射组细胞形态改变轻于模型组;静脉注射组、关节腔注射组之间形态结构相似。结论:云克针剂关节腔内注射与静脉注射均能对兔膝骨关节炎有治疗作用,能有效延缓关节软骨退变进程。

Objective : To observe the pathological change of cartilage by injection of 99TC - MDP into knee - joints of rabbitwith knee osteoarthritis ( KOA ) .Methods : All 32male New Zealand big - eared white rabbits were randomly divided intonormal group , model group , intravenous injection groupand intraarticular injection group.No intervention was imposedon the normal group , while other groups received the treatment of the first 1/ 3of the medial meniscus resection of therabbit knee and the anterior cruciate ligament transaction ( ACLT ) .No other treatment on the normal group and modelgroup.The intravenous injection group was daily given 99TC - MDP injection of 0.2mL into rabbit vein for 5weeks.Theintraarticular injection group was given 99TC - MDP injection of 0.2mL into rabbit knee- joints once a week for 5weeks.At the sixth week , the pathological changes were observed under the microscope.Results : HE stains showed that normalgroup was better than the other three groups in Mankin scores.The intravenous injection groupand the intraarticular in -jection group was better than the model groupin Mankin scores.There was no statistically significant difference betweenthe intravenous injection group and the intraarticular injection group.Ultrastructure of cartilage cells of the two groupstreated by 99TC - MDP was better than the model group.There were no differences between the intravenous injectiongroup and the intraarticular injection group.Conclusion: Injection of 99TC - MDP into rabbit knee- joints can be used totreat rabbit osteoarthritis and effectively delay the process of articular cartilage degeneration.